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G protein subunit G alpha 16 expression is restricted to progenitor B cells
during human B-cell differentiation
MY Mapara, K Bommert, RC Bargou, C Leng, C Beck, WD Ludwig, P Gierschik and B Dorken
Free University of Berlin, University Medical Center Rudolf Virchow, Robert
Rossle Klinik, Department of Internal Medicine, Germany.
Recently G alpha 16, a new guanosine triphosphate (GTP) binding protein
alpha subunit has been described to be specifically expressed in human
hematopoietic cells. Expression of G alpha 16 was observed in human cell
lines of myelomonocytic and T-lymphocytic origin, but not in human B-cell
lines Raji and IM9. We studied the expression of G alpha 16 in human B
cells corresponding to different stages of B-cell differentiation by means
of reverse transcriptase-polymerase chain reaction (RT-PCR) and Western
blotting. The human Burkitt's lymphoma cell lines Raji, Ramos, BJAB, the
lymphoblastoid cell line SKW6.4, and the plasmocytoma cell line U266 were
devoid of G alpha 16. In contrast, G alpha 16 was detected in the human
progenitor B cell lines Reh and Nalm-6. Using the mu+, k- cell line BLIN-1
(pre-B cell phenotype) and its derived subclone 1E8 (surface mu+, k+;
B-cell phenotype) G alpha 16 expression was found to disappear on
transition from pre-B to B-cell differentiation stage. The analysis of a
broad panel of human neoplastic B lymphocytes ranging from progenitor
B-acute lymphatic leukemia (pre-pre-B-ALL), common acute leukemias (cALL),
pre-B-ALL, mature B-ALL to low grade B-cell lymphoma (chronic lymphocytic
leukemia of B-cell type, leukemic centrocytic non-Hodgkins lymphoma [NHL],
hairy cell leukemia) showed that G alpha 16 expression is limited to
progenitor and pre-B-ALL cells. Therefore, we conclude that within B- cell
differentiation, G alpha 16 is expressed solely during early B cell
ontogeny and downregulated during differentiation. Thus, G alpha 16 might
be an important regulator involved in signaling processes in progenitor B
cells.
Volume 85,
Issue 7,
pp. 1836-1842,
04/01/1995
Copyright © 1995 by The American Society of Hematology
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