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Interleukin-3 and interleukin-7 are alternative growth factors for the same
B-cell precursors in the mouse
TH Winkler, F Melchers and AG Rolink
Basel Institute for Immunology, Switzerland.
Clones and lines of precursor (pre) B cells can be established by limiting
dilutions of unseparated cell suspensions of fetal liver or bone marrow on
stromal cells in the presence of interleukin (IL)-7. When IL-3 is used
instead of IL-7, cultures are regularly overgrown by different precursor
cells of the myeloid lineage, as well as by adherent cells that inhibit
pre-B-cell expansion. However, in the presence of either IL-7 or IL-3,
clones of pre-B cells can be established on stroma cells at frequencies
near one in one when the cultures are initiated with cell sorter purified
CD45RO (B220)+/c-kit+ fetal liver or bone marrow derived pre-B cells.
Clones grown on stromal cells in the presence of IL-7 can be regrown in
IL-3, and vice versa. Pre-B cells that proliferate on stromal cells in the
presence of IL-7 or IL-3 have the same phenotype, ie, are B220+ c-kit+,
CD43+, and surrogate light chain+. Removal of the growth factors (IL-7,
respectively IL-3) from the cultures results in differentiation to surface
immunoglobulin (slg) positive, c-kit-, CD43-, surrogate light chain- B
cells, a fraction of which is lipopolysaccharide (LPS) responsive as shown
by IgM secretion. These results show that IL-7 and IL-3 stimulate largely
overlapping populations of precursor B cells from bone marrow to
proliferate for long periods of time in the presence of stromal cells.
Thus, IL-7 and IL-3 are alternative growth factors for the same pre-BI
cell.
Volume 85,
Issue 8,
pp. 2045-2051,
04/15/1995
Copyright © 1995 by The American Society of Hematology
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