|
|
 Previous Article | Table of Contents | Next Article 
Identification and characterization of a soluble c-kit receptor produced by
human hematopoietic cell lines
AM Turner, LG Bennett, NL Lin, J Wypych, TD Bartley, RW Hunt, HL Atkins, KE Langley, V Parker and F Martin
Division of Hematology, University of Washington, Seattle 98195, USA.
Stem cell factor (SCF) triggers cell growth by binding to cell surface
c-kit receptors. Soluble forms of several cytokine receptors have been
described and may play a role in the modulation of cytokine activity in
vivo. For these reasons, we investigated whether human hematopoietic cells
produce soluble c-kit receptors. The human leukemia cell lines OCIM1 and
MO7e display approximately 80,000 and approximately 35,000 high-affinity
cell surface c-kit receptors, respectively. Soluble c-kit receptors were
detected by enzyme immunoassay in OCIM1 and MO7e culture supernatants. We
determined the molecular weight and binding affinity of soluble c-kit
receptor produced by OCIM1 cells, soluble c-kit receptor purified from
human serum, and recombinant soluble c-kit receptor expressed in CHO cells.
The three soluble c-kit receptors each have a molecular weight of 98 kD.
Quantitative binding experiments with 125I-SCF indicate that the soluble
c-kit receptors obtained from human serum or OCIM1 cells have binding
affinities for SCF of approximately 200 to 300 pmol/L, in contrast to the
recombinant form, which has a binding affinity of approximately 1.5 nmol/L.
All three forms of the soluble c-kit receptor were able to compete with
c-kit receptors on OCIM1 cells for 125I-SCF binding. Thus human
hematopoietic cells can produce a soluble form of the c-kit receptor that
retains high-affinity SCF binding activity. We speculate that the soluble
c-kit receptor may bind SCF and function as a receptor antagonist in vivo.
Volume 85,
Issue 8,
pp. 2052-2058,
04/15/1995
Copyright © 1995 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
S. E. DePrimo, X. Huang, M. E. Blackstein, C. R. Garrett, C. S. Harmon, P. Schoffski, M. H. Shah, J. Verweij, C. M. Baum, and G. D. Demetri
Circulating Levels of Soluble KIT Serve as a Biomarker for Clinical Outcome in Gastrointestinal Stromal Tumor Patients Receiving Sunitinib following Imatinib Failure
Clin. Cancer Res.,
September 15, 2009;
15(18):
5869 - 5877.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K.J. Hutt, E.A. McLaughlin, and M.K. Holland
Kit ligand and c-Kit have diverse roles during mammalian oogenesis and folliculogenesis
Mol. Hum. Reprod.,
February 1, 2006;
12(2):
61 - 69.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Akin, L. B. Schwartz, T. Kitoh, H. Obayashi, A. S. Worobec, L. M. Scott, and D. D. Metcalfe
Soluble stem cell factor receptor (CD117) and IL-2 receptor alpha chain (CD25) levels in the plasma of patients with mastocytosis: relationships to disease severity and bone marrow pathology
Blood,
August 15, 2000;
96(4):
1267 - 1273.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. D. Lyman and S. E. W. Jacobsen
c-kit Ligand and Flt3 Ligand: Stem/Progenitor Cell Factors With Overlapping Yet Distinct Activities
Blood,
February 15, 1998;
91(4):
1101 - 1134.
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. C. Broudy, N. L. Lin, H.-J. Buhring, N. Komatsu, and T. J. Kavanagh
Analysis of c-kit Receptor Dimerization by Fluorescence Resonance Energy Transfer
Blood,
February 1, 1998;
91(3):
898 - 906.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. C. Broudy
Stem Cell Factor and Hematopoiesis
Blood,
August 15, 1997;
90(4):
1345 - 1364.
[Full Text]
[PDF]
|
|
|
|
|
