|
|
 Previous Article | Table of Contents | Next Article 
Identification of signaling motifs within human Fc gamma RIIa and Fc gamma
RIIb isoforms
IE Van den Herik-Oudijk, PJ Capel, T van der Bruggen and JG Van de Winkel
Department of Immunology, University Hospital Utrecht, The Netherlands.
To assess the functional capacity of the heterogeneous Fc gamma RII (CD32)
family and to identify critical regions for functioning, we generated a
panel of B-cell transfectants. The Fc gamma R-negative B- cell line IIA1.6
was transfected with wild-type or mutant human Fc gamma RIIa and IIb
molecules. Solely Fc gamma RIIa-expressing IIA1.6 cells were capable of
phagocytosing opsonized Staphylococcus aureus bacteria, and cross-linking
of Fc gamma RIIa triggered a rapid induction of tyrosine phosphorylation
after 20 seconds. Analysis of Fc gamma RIIa mutants identified the
immunoreceptor tyrosine-based activation motif (ITAM; previously described
as ARH-1 motif) within the IIa cytoplasmic tail to be critical for B-cell
activation. In contrast, Fc gamma RIIb isoforms triggered tyrosine
phosphorylation on cross- linking with much slower kinetics (> 3
minutes) than Fc gamma RIIa. Furthermore, solely Fc gamma RIIb molecules
proved capable of downregulating [Ca2+]i and interleukin-2 production on
co-cross-linking with sIgG in IIA1.6. The Fc gamma RIIb-mediated functions
were absent in Fc gamma RIIb mutants in which the tyrosine or leucine
within the YSLL motif in a conserved 13-aa region (now known as
immunoreceptor tyrosine-based inhibitor motif [ITIM]) were changed into
phenylalanines. In conclusion, these data show the presence of functionally
critical motifs within Fc gamma RII cytoplasmic tails. Fc gamma RIIa
contains an ITAM involved in B-cell activatory functions, whereas the
downregulatory activity of Fc gamma RIIb isoforms is linked to an ITIM.
Volume 85,
Issue 8,
pp. 2202-2211,
04/15/1995
Copyright © 1995 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
G. Berntzen, J. T. Andersen, K. Ustgard, T. E. Michaelsen, S. A. Mousavi, J. D. Qian, P. E. Kristiansen, V. Lauvrak, and I. Sandlie
Identification of a High Affinity Fc{gamma}RIIA-binding Peptide That Distinguishes Fc{gamma}RIIA from Fc{gamma}RIIB and Exploits Fc{gamma}RIIA-mediated Phagocytosis and Degradation
J. Biol. Chem.,
January 9, 2009;
284(2):
1126 - 1135.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Boylan, C. Gao, V. Rathore, J. C. Gill, D. K. Newman, and P. J. Newman
Identification of Fc{gamma}RIIa as the ITAM-bearing receptor mediating {alpha}IIb{beta}3 outside-in integrin signaling in human platelets
Blood,
October 1, 2008;
112(7):
2780 - 2786.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. A. Otten, J. H. W. Leusen, E. Rudolph, J. A. van der Linden, R. H. J. Beelen, J. G. J. van de Winkel, and M. van Egmond
FcR {gamma}-Chain Dependent Signaling in Immature Neutrophils Is Mediated by Fc{alpha}RI, but Not by Fc{gamma}RI
J. Immunol.,
September 1, 2007;
179(5):
2918 - 2924.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z.-Y. Huang, D. R. Barreda, R. G. Worth, Z. K. Indik, M.-K. Kim, P. Chien, and A. D. Schreiber
Differential kinase requirements in human and mouse Fc-gamma receptor phagocytosis and endocytosis
J. Leukoc. Biol.,
December 1, 2006;
80(6):
1553 - 1562.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Monari, T. R. Kozel, F. Paganelli, E. Pericolini, S. Perito, F. Bistoni, A. Casadevall, and A. Vecchiarelli
Microbial Immune Suppression Mediated by Direct Engagement of Inhibitory Fc Receptor
J. Immunol.,
November 15, 2006;
177(10):
6842 - 6851.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. W. S. I. Rodrigo, X. Jin, S. D. Blackley, R. C. Rose, and J. J. Schlesinger
Differential Enhancement of Dengue Virus Immune Complex Infectivity Mediated by Signaling-Competent and Signaling-Incompetent Human Fc{gamma}RIA (CD64) or Fc{gamma}RIIA (CD32).
J. Virol.,
October 1, 2006;
80(20):
10128 - 10138.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. van Mirre, W. B. Breunis, J. Geissler, C. E. Hack, M. de Boer, D. Roos, and T. W. Kuijpers
Neutrophil responsiveness to IgG, as determined by fixed ratios of mRNA levels for activating and inhibitory Fc{gamma}RII (CD32), is stable over time and unaffected by cytokines
Blood,
July 15, 2006;
108(2):
584 - 590.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. F. Rosse, P. Hillmen, and A. D. Schreiber
Immune-Mediated Hemolytic Anemia
Hematology,
January 1, 2004;
2004(1):
48 - 62.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. P. Ganesan, H. Fang, C. B. Marsh, and S. Tridandapani
The Protein-tyrosine Phosphatase SHP-1 Associates with the Phosphorylated Immunoreceptor Tyrosine-based Activation Motif of Fc{gamma}RIIa to Modulate Signaling Events in Myeloid Cells
J. Biol. Chem.,
September 12, 2003;
278(37):
35710 - 35717.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Tridandapani, R. Wardrop, C. P. Baran, Y. Wang, J. M. Opalek, M. A. Caligiuri, and C. B. Marsh
TGF-{beta}1 Suppresses Myeloid Fc{gamma} Receptor Function by Regulating the Expression and Function of the Common {gamma}-Subunit
J. Immunol.,
May 1, 2003;
170(9):
4572 - 4577.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Tridandapani, Y. Wang, C. B. Marsh, and C. L. Anderson
Src Homology 2 Domain-Containing Inositol Polyphosphate Phosphatase Regulates NF-{kappa}B-Mediated Gene Transcription by Phagocytic Fc{gamma}Rs in Human Myeloid Cells
J. Immunol.,
October 15, 2002;
169(8):
4370 - 4378.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. E. Rodriguez, S. M. M. Hellwig, D. F. Hozbor, J. Leusen, W. -L. van der Pol, and J. G. J. van de Winkel
Fc Receptor-Mediated Immunity Against Bordetella pertussis
J. Immunol.,
December 1, 2001;
167(11):
6545 - 6551.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. H. Lee, M. Ono, M. Inui, T. Yuasa, and T. Takai
Stimulatory Function of gp49A, a Murine Ig-Like Receptor, in Rat Basophilic Leukemia Cells
J. Immunol.,
November 1, 2000;
165(9):
4970 - 4977.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Pricop, J. Gokhale, P. Redecha, S. C. Ng, and J. E. Salmon
Reactive Oxygen Intermediates Enhance Fc{gamma} Receptor Signaling and Amplify Phagocytic Capacity
J. Immunol.,
June 15, 1999;
162(12):
7041 - 7048.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. van Egmond, A.J. H. van Vuuren, H. C. Morton, A. B. van Spriel, L. Shen, F. M.A. Hofhuis, T. Saito, T. N. Mayadas, J. S. Verbeek, and J. G.J. van de Winkel
Human Immunoglobulin A Receptor (Fcalpha RI, CD89) Function in Transgenic Mice Requires Both FcR gamma Chain and CR3 (CD11b/CD18)
Blood,
June 15, 1999;
93(12):
4387 - 4394.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. C. Baiu, J. Prechl, A. Tchorbanov, H. D. Molina, A. Erdei, A. Sulica, P. J. A. Capel, and W. L. W. Hazenbos
Modulation of the Humoral Immune Response by Antibody-Mediated Antigen Targeting to Complement Receptors and Fc Receptors
J. Immunol.,
March 15, 1999;
162(6):
3125 - 3130.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Hunter, Z. K. Indik, M.-K. Kim, M. D. Cauley, J.-G. Park, and A. D. Schreiber
Inhibition of Fcgamma Receptor-Mediated Phagocytosis by a Nonphagocytic Fcgamma Receptor
Blood,
March 1, 1998;
91(5):
1762 - 1768.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. T. Lu, M. Barreuther, S. Davis, and J. A. Madri
Platelet Endothelial Cell Adhesion Molecule-1 Is Phosphorylatable by c-Src, Binds Src-Src homology 2 Domain, and Exhibits Immunoreceptor Tyrosine-based Activation Motif-like Properties
J. Biol. Chem.,
May 30, 1997;
272(22):
14442 - 14446.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. C. Morton, I. E. van den Herik-Oudijk, P. Vossebeld, A. Snijders, A. J. Verhoeven, P. J. A. Capel, and J. G. J. van de Winkel
Functional Association between the Human Myeloid Immunoglobulin A Fc Receptor (CD89) and FcR [IMAGE] Chain
J. Biol. Chem.,
December 15, 1995;
270(50):
29781 - 29787.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
