Interleukin-11 stimulates multilineage progenitors, but not stem cells, in
murine and human long-term marrow cultures
XX Du, D Scott, ZX Yang, R Cooper, XL Xiao and DA Williams
Herman B Wells Center for Pediatric Research, James Whitcomb Riley Hospital
for Children, Indiana University School of Medicine, Indianapolis
46202-5225, USA.
Interleukin-11 (IL-11) is a bone marrow microenvironment-derived growth
factor with pleiotropic effects on a variety of hematopoietic cells. To
more accurately assess the effects of IL-11 on stem and progenitor
compartments within the hematopoietic microenvironment (HM), we added
recombinant human (rh) IL-11 to human and murine long-term bone marrow
cultures (LTMC) and analyzed primitive (high proliferative potential-
colony forming cells [HPP-CFC], long-term culture-initiating cells [LTC-
IC], and long-term reconstituting stem cells) and progenitor (day 12 colony
forming unit-spleen [CFU-S12], colony forming unit-megakaryocyte [CFU-Mk]
and colony forming unit-granulocyte/macrophage [CFU-GM]) compartments
throughout the duration of the cultures. rhIL-11 (100 ng/mL) added twice
weekly resulted in significantly increased nonadherent (NA) cellularity,
CFU-GM, and CFU-Mk production in human LTMC. Addition of rhIL-11 to murine
LTMC was associated with a 5- to 40- fold increase in CFU-GM and a four- to
20-fold increase in day 12 CFU-S in NA cells. However, IL-11 had no
significant effect on total HPP-CFC concentration and decreased the size of
the more primitive stem/progenitor compartment as evidenced by both
decreased LTC-IC frequency in human LTMC and decreased frequency of
long-term reconstituting stem cells in murine LTMC. These data suggest that
IL-11 may increase commitment of stem cells into a multipotential
progenitor compartment.
Volume 86,
Issue 1,
pp. 128-134,
07/01/1995
Copyright © 1995 by The American Society of Hematology
