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The human leukocyte integrin CD11a promoter directs expression in
leukocytes of transgenic mice
KA Ritchie, A Aprikian, KA Gollahon and DD Hickstein
Medical Research Service, Seattle Veterans Affairs Medical Center, WA
98108, USA.
The human CD11a molecule is expressed specifically on lymphocytes,
monocyte/macrophages, and neutrophils, in which it mediates important
adhesion-related functions. We used 1.7 kb of regulatory sequences upstream
from the human CD11a gene transcription start site to drive expression of a
modified human CD4 reporter gene in transgenic mice. The transgene was
expressed in a tissue-specific fashion on all leukocytes and paralleled
endogenous mouse CD11a expression. All five founder mice expressed the
transgene, providing evidence for integration site-independent expression.
However, expression was not proportional to transgene copy number. These
studies indicate that (1) the mutated human CD4 serves as an excellent
reporter for analysis of leukocyte-specific promoters; (2) the CD11a
regulatory unit used here represents a novel reagent for targeting gene
expression to leukocytes; and (3) additional regulatory regions will be
required for copy-number- dependent activity of CD11a regulatory sequences.
Volume 86,
Issue 1,
pp. 147-155,
07/01/1995
Copyright © 1995 by The American Society of Hematology
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