|
|
 Previous Article | Table of Contents | Next Article 
Retinoic acid reduces induction of monocyte tissue factor and tissue
factor/factor VIIa-dependent arterial thrombus formation
RM Barstad, MJ Hamers, RW Stephens and KS Sakariassen
Nycomed Bioreg AS, Oslo, Norway.
Agents that downregulate the induction of monocyte/macrophage tissue factor
(TF) activity may attenuate the thrombotic risk associated with mechanical
restoration of vessel patency or artificial arterial grafting. In such
events, procoagulant macrophages in the atherosclerotic plaque and
procoagulant monocytes adherent to artificial materials may be exposed to
the blood stream. Ishii et al (Blood 80:2556, 1992) reported that induction
of endothelial TF is downregulated by all-trans retinoic acid (ATRA), and
Conese et al (Thromb Haemost 66:662, 1991) reported that retinoids
downregulate monocyte procoagulant activity (PCA). These findings led us to
investigate the effect of ATRA on monocyte TF expression, and to study the
effect of ATRA on monocyte-induced thrombus formation in a model system of
human arterial thrombogenesis. Induction of PCA in human peripheral blood
monocytes by 0.5 microgram/mL lipopolysaccharide (LPS) was dose dependently
reduced by ATRA, reaching a reduction of 56% at 10(-5) mol/L ATRA (P <
.0001). A 38% reduction (P < .0007) in LPS- induced TF antigen
expression was observed at an ATRA concentration of 10(-6) mol/L. Adherence
of monocytes to plastic cover slips (Thermanox, Miles Laboratories,
Naperville, IL) also triggered induction of cellular PCA, which was
inhibited by more than 80% by an anti-TF monoclonal antibody (MoAb) (P <
.002). Inclusion of ATRA (10(-6) mol/L) reduced this PCA by 40% (P <
.03), and the TF antigen expression by 30% (P < .0001). Exposure of
Thermanox adherent monocytes to flowing nonanticoagulated human blood in a
parallel-plate perfusion chamber device at an arterial wall shear rate of
650 s-1 elicited significant fibrin deposition and platelet thrombus
formation. Partial interruption of this thrombus formation was achieved by
10(-6) mol/L ATRA, which reduced the fibrin deposition by 80% (P < .02)
and platelet thrombus formation by 50% (P < .05). In comparison,
incubation of adherent monocytes with the anti-TF MoAb before the blood
exposure, reduced the fibrin deposition by 83% (P < .02) and platelet
thrombus volume by 75% (P < .0008). Thus, ATRA is an effective
down-regulator of monocyte TF- PCA, and may reduce thrombotic complications
at sites of plaque rupture, at plaque disruption after percutaneous
transluminal angioplasty procedures, or on surfaces introduced by
artificial arterial grafting.
Volume 86,
Issue 1,
pp. 212-218,
07/01/1995
Copyright © 1995 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
M. Camacho, C. Rodriguez, J. Salazar, J. Martinez-Gonzalez, J. Ribalta, J.-R. Escudero, L. Masana, and L. Vila
Retinoic acid induces PGI synthase expression in human endothelial cells
J. Lipid Res.,
August 1, 2008;
49(8):
1707 - 1714.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Fujiu, I. Manabe, A. Ishihara, Y. Oishi, H. Iwata, G. Nishimura, T. Shindo, K. Maemura, H. Kagechika, K. Shudo, et al.
Synthetic Retinoid Am80 Suppresses Smooth Muscle Phenotypic Modulation and In-Stent Neointima Formation by Inhibiting KLF5
Circ. Res.,
November 25, 2005;
97(11):
1132 - 1141.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. I Axel, A. Frigge, J. Dittmann, H. Runge, I. Spyridopoulos, R. Riessen, R. Viebahn, and K. R Karsch
All-trans retinoic acid regulates proliferation, migration, differentiation, and extracellular matrix turnover of human arterial smooth muscle cells
Cardiovasc Res,
March 1, 2001;
49(4):
851 - 862.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. P. Neve, D. Corseaux, G. Chinetti, C. Zawadzki, J.-C. Fruchart, P. Duriez, B. Staels, and B. Jude
PPAR{{alpha}} Agonists Inhibit Tissue Factor Expression in Human Monocytes and Macrophages
Circulation,
January 16, 2001;
103(2):
207 - 212.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Ohsawa, T. Koyama, K. Yamamoto, S. Hirosawa, S. Kamei, and R. Kamiyama
1{alpha},25-Dihydroxyvitamin D3 and Its Potent Synthetic Analogs Downregulate Tissue Factor and Upregulate Thrombomodulin Expression in Monocytic Cells, Counteracting the Effects of Tumor Necrosis Factor and Oxidized LDL
Circulation,
December 5, 2000;
102(23):
2867 - 2872.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. M. Miano and B. C. Berk
Retinoids : Versatile Biological Response Modifiers of Vascular Smooth Muscle Phenotype
Circ. Res.,
September 1, 2000;
87(5):
355 - 362.
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Neuville, M.-L. Bochaton-Piallat, and G. Gabbiani
Retinoids and Arterial Smooth Muscle Cells
Arterioscler Thromb Vasc Biol,
August 1, 2000;
20(8):
1882 - 1888.
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. J. Wiegman, W. L. Barry, J. A. McPherson, C. A. McNamara, L. W. Gimple, J. M. Sanders, G. G. Bishop, E. R. Powers, M. Ragosta, G. K. Owens, et al.
All-trans-Retinoic Acid Limits Restenosis After Balloon Angioplasty in the Focally Atherosclerotic Rabbit : A Favorable Effect on Vessel Remodeling
Arterioscler Thromb Vasc Biol,
January 1, 2000;
20(1):
89 - 95.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Koyama, M. Shibakura, M. Ohsawa, R. Kamiyama, and S. Hirosawa
Anticoagulant Effects of 1alpha ,25-Dihydroxyvitamin D3 on Human Myelogenous Leukemia Cells and Monocytes
Blood,
July 1, 1998;
92(1):
160 - 167.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Oeth, J. Yao, S.-T. Fan, and N. Mackman
Retinoic Acid Selectively Inhibits Lipopolysaccharide Induction of Tissue Factor Gene Expression in Human Monocytes
Blood,
April 15, 1998;
91(8):
2857 - 2865.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Kaikita, H. Ogawa, H. Yasue, M. Takeya, K. Takahashi, T. Saito, K. Hayasaki, K. Horiuchi, A. Takizawa, Y. Kamikubo, et al.
Tissue Factor Expression on Macrophages in Coronary Plaques in Patients with Unstable Angina
Arterioscler Thromb Vasc Biol,
October 1, 1997;
17(10):
2232 - 2237.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
M. Shibakura, T. Koyama, T. Saito, K. Shudo, N. Miyasaka, R. Kamiyama, and S. Hirosawa
Anticoagulant Effects of Synthetic Retinoids Mediated Via Different Receptors on Human Leukemia and Umbilical Vein Endothelial Cells
Blood,
August 15, 1997;
90(4):
1545 - 1551.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
