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BCL-6 gene product, a 92- to 98-kD nuclear phosphoprotein, is highly
expressed in germinal center B cells and their neoplastic counterparts
T Onizuka, M Moriyama, T Yamochi, T Kuroda, A Kazama, N Kanazawa, K Sato, T Kato, H Ota and S Mori
Department of Pathology, University of Tokyo, Japan.
The BCL-6 gene is known to be located on chromosome 3q27, at the breakpoint
of the 3q27-associated translocations that occur frequently in human
non-Hodgkin's lymphomas (NHLs). To identify the BCL-6 protein, two
antibodies that recognized distinct domains of this protein were raised in
rabbits. Immunoprecipitation and immunoblotting of lysates of
BCL-6-expressing cells using both antibodies showed a broad 92- to 98- kD
band. Dephosphorylation of BCL-6 protein reduced the size of this band to
87 kD, suggesting that BCL-6 may be expressed in a phosphorylated form.
Immunostaining with both antibodies showed that BCL-6 protein was localized
in the nuclei of most of the germinal center B cells and a small number of
marginal zone B cells. Furthermore, BCL-6 protein was expressed in
follicular, Burkitt's, and diffuse large B-cell lymphomas. These results
suggest that the BCL-6 protein, expressed in B cells of the germinal
centers which are important in the maturation of immune responses, may play
some physiological role(s) in the germinal center B cells.
Volume 86,
Issue 1,
pp. 28-37,
07/01/1995
Copyright © 1995 by The American Society of Hematology
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