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Accelerated cell-cycling of hematopoietic progenitor cells by growth
factors
R Tanaka, N Katayama, K Ohishi, N Mahmud, R Itoh, Y Tanaka, Y Komada, N Minami, M Sakurai and S Shirakawa
Department of Pediatrics, Mie University School of Medicine, Mie University
Hospital, Japan.
Recent advances in molecular biology have led to the identification of
hematopoietic growth factors that support and influence the proliferation
of hematopoietic progenitor cells in vitro and in vivo. Although these
factors have been extensively studied, little is known of their role in the
regulation of cell-cycling of hematopoietic progenitors, especially in the
early stage of hematopoiesis. In the present study, we examined the effects
of early acting growth factors on proliferative kinetics of hematopoietic
progenitors by monitoring the number of cells in individual developing
colonies, using an in vitro clonal assay. Interleukin-11 (IL-11) or steel
factor (SF), alone or in combination, shortened the time for the size of
IL-3-dependent colonies to double. Consecutive replating experiments
provided evidence for direct action of growth factors on the growth rate of
hematopoietic progenitor cells. Shortening of the time for the total cell
number in the colonies to double was due to a reduction in time for each
single cell within the respective colonies to become two daughter cells,
and there was no alteration in the incidence of cells with a proliferative
capacity. Cell-cycle analysis demonstrated that IL-11 has the potential to
induce a shortened time for cell-cycle of hematopoietic progenitor cells
without affecting distribution of each fraction of the cell- cycle, whereas
SF has the potential to reduce cell-cycle time mainly by decreasing the
time required for hematopoietic progenitor cells to go through the G1
phase. These results suggest that growth factors may modulate cell-cycling
of hematopoietic progenitor cells.
Volume 86,
Issue 1,
pp. 73-79,
07/01/1995
Copyright © 1995 by The American Society of Hematology
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