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This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Eipers, P. Articles by Clarke, M. Search for Related Content PubMed PubMed Citation Articles by Eipers, P. Articles by Clarke, M. Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Retroviral-mediated gene transfer in human bone marrow cells growth in continuous perfusion culture vessels PG Eipers, JC Krauss, BO Palsson, SG Emerson, RF Todd and MF Clarke Department of Internal Medicine, University of Michigan, Ann Arbor 48109-0668, USA. Hematopoietic stem cell gene therapy holds the promise of being able to treat a variety of inherited and acquired diseases of the hematopoietic stem cell. However, to date, genetic modification of the human hematopoietic stem cell has been relatively inefficient. Here, we report the results of using a bioreactor system to expand hematopoietic cells after a brief retrovirus infection using a high titer, replication defective virus encoding for murine CD18. The retrovirus transduced culture continued to produce genetically modified hematopoietic progenitors for up to 6 weeks, the duration of the culture period. Up to one-third of the long-term culture initiating cell (LTC-IC) are genetically modified by the culture conditions. Murine CD18 can be expressed on the cell surface of up to 20% of the mature cells generated by the culture system, suggesting that clinically significant levels of gene transfer may be occurring. These results demonstrate the feasibility of using continuous perfusion bioreactors as a method of efficiently modifying human hematopoietic stem cells. Volume 86, Issue 10, pp. 3754-3762, 11/15/1995 Copyright © 1995 by The American Society of Hematology CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: E. C. MacNeill, H. Hanenberg, K. E. Pollok, J. C. M. van der Loo, M. F. A. Bierhuizen, G. Wagemaker, and D. A. Williams Simultaneous Infection with Retroviruses Pseudotyped with Different Envelope Proteins Bypasses Viral Receptor Interference Associated with Colocalization of gp70 and Target Cells on Fibronectin CH-296 J. Virol., May 1, 1999; 73(5): 3960 - 3967. [Abstract] [Full Text]

	Copyright © 1995 by American Society of Hematology         Online ISSN: 1528-0020