|
|
 Previous Article | Table of Contents | Next Article 
Separation of myeloid and erythroid progenitors based on expression of CD34
and c-kit
MO De Jong, G Wagemaker and AW Wognum
Institute of Hematology, Erasmus University, Rotterdam, The Netherlands.
In this report, a novel approach is described to physically separate
erythroid progenitors from monocyte and granulocyte progenitors, based on
the expression of CD34 and Kit. Using biotin-labeled human Kit ligand (KL)
and flow cytometry, Kit was detectable on 2% to 3% of the nucleated cells
in rhesus monkey bone marrow. Combination of biotin-KL with CD34 monoclonal
antibodies (MoAb) showed that Kit was expressed on subsets of CD34low and
CD34pos cells. Our data clearly demonstrate that CD34pos cells are more
heterogeneous with respect to Kit expression than observed in studies using
Kit MoAb. A small cluster, approximately 7% of the CD34pos cells, expressed
CD34 at submaximal levels and stained brightly with biotinylated KL. This
CD34pos/kithi fraction contained predominantly erythroid progenitors
(burst-forming units- erythroid; BFU-E). The majority of the granulocytic
and monocytic progenitors (colony-forming units-granulocyte/macrophage;
CFU-GM) were CD34pos/kitmed. Some BFU-E were also detected in the
CD34pos/kitmed and CD34low/kitpos fractions at low frequency. In the latter
subset, most erythroid colony-forming units (CFU-E) were recovered. Using
three- color flow cytometry, we analyzed expression of Kit in relation to
that of CD34 and the class II major histocompatibility antigen, RhLA-DR.
The most immature bone marrow cells that can be identified in vitro, ie,
CD34pos/RhLA-DRlow cells, were kitmed. The CD34pos/kithi and CD34pos/kitneg
subsets predominantly contained the more mature RhLA- DRbright cells. Our
results demonstrate that erythroid precursors express c-kit at much higher
levels than monomyeloid precursors and pluripotent progenitors. The
difference in expression levels of CD34 and c-kit can be exploited to
isolate BFU-E populations that are virtually devoid of nonerythroid cells.
Volume 86,
Issue 11,
pp. 4076-4085,
12/01/1995
Copyright © 1995 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
E. A. de Wynter, C. M. Heyworth, N. Mukaida, E. Jaworska, A. Weffort-Santos, K. Matushima, and N. G. Testa
CCR1 chemokine receptor expression isolates erythroid from granulocyte-macrophage progenitors
J. Leukoc. Biol.,
September 1, 2001;
70(3):
455 - 460.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Ikehara
Pluripotent Hemopoietic Stem Cells in Mice and Humans
Experimental Biology and Medicine,
February 1, 2000;
223(2):
149 - 155.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
L. Lu, M. C. Heinrich, L.-S. Wang, M.-S. Dai, A. J. Zigler, L. Chai, and H. E. Broxmeyer
Retroviral-Mediated Gene Transduction of c-kit Into Single Hematopoietic Progenitor Cells From Cord Blood Enhances Erythroid Colony Formation and Decreases Sensitivity to Inhibition by Tumor Necrosis Factor-alpha and Transforming Growth Factor-beta 1
Blood,
October 1, 1999;
94(7):
2319 - 2332.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. C. Broudy
Stem Cell Factor and Hematopoiesis
Blood,
August 15, 1997;
90(4):
1345 - 1364.
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Inada, A. Iwama, S. Sakano, M. Ohno, K.-i. Sawada, and T. Suda
Selective Expression of the Receptor Tyrosine Kinase, HTK, on Human Erythroid Progenitor Cells
Blood,
April 15, 1997;
89(8):
2757 - 2765.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
