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Detection of cytomegalovirus DNA in CD34+ cells from blood and bone marrow
D von Laer, U Meyer-Koenig, A Serr, J Finke, L Kanz, AA Fauser, D Neumann- Haefelin, W Brugger and FT Hufert
Abteilung Virologie, Universitat Freiburg, Germany.
Infection of hematopoietic progenitor cells with the human cytomegalovirus
(HCMV) has been proposed as an explanation for the cytopenias associated
with HCMV-related disease. To test this hypothesis, CD34+ cells, which
include the hematopoietic progenitors, as well as mature leukocyte
populations were purified on a fluorescence- activated cell sorter and
analyzed for HCMV DNA by polymerase chain reaction (PCR). A total of 33
samples from 31 immunosuppressed as well as immunocompetent
HCMV-seropositive individuals were studied. CD34+ cells were PCR-positive
in four of seven bone marrow aspirates from allogeneic bone marrow
transplant recipients, in three of eight aspirates from patients with
acquired immunodeficiency syndrome, and in the first of two bone marrow
samples from an immunocompetent patient with primary HCMV disease. CD34+
cells purified from peripheral blood for autologous and allogeneic
transplantation were also analyzed, and 4 of 13 samples were HCMV
DNA-positive. Interestingly, two of the four HCMV-positive samples were
from healthy allogeneic donors. Among the mature leukocyte populations, the
monocytes were most frequently found to be HCMV DNA-positive. No HCMV DNA
was detected in the total bone marrow leukocytes of 13 healthy seropositive
bone marrow donors or in the CD34+ cell fraction of three further
seropositive donors. In conclusion, the data provide strong evidence that
CD34+ hematopoietic progenitor cells can be infected with HCMV in
immunosuppressed patients, while this cell population was not identified as
a major viral reservoir in healthy HCMV-seropositive individuals.
Volume 86,
Issue 11,
pp. 4086-4090,
12/01/1995
Copyright © 1995 by The American Society of Hematology
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