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Plasma/serum levels of flt3 ligand are low in normal individuals and highly
elevated in patients with Fanconi anemia and acquired aplastic anemia
SD Lyman, M Seaberg, R Hanna, J Zappone, K Brasel, JL Abkowitz, JT Prchal, JC Schultz and NT Shahidi
Immunex Corporation, Seattle, WA 98101, USA.
The flt3 ligand is a growth factor that stimulates the proliferation of
hematopoietic progenitor and stem cells. We established a sensitive
enzyme-linked immunosorbent assay (ELISA) to measure the concentration of
flt3 ligand in plasma or serum from normal individuals, as well as in
patients with hematopoietic disorders. Concentrations of flt3 ligand in
plasma or serum from normal individuals were quite low: only 12% (7 of 60)
of normal individuals had flt3 ligand levels above 100 pg/mL (the limit of
detection). In contrast, 86% (19 of 22) of samples from patients with
Fanconi anemia and 100% (eight of eight) of samples from patients with
acquired aplastic anemia had plasma or serum levels above 100 pg/mL. Mean
plasma or serum concentrations (calculated by assigning a value of 0 pg/mL
to any sample reading below the level of detection) were as follows: normal
volunteers, 14 pg/mL; patients with Fanconi anemia, 1,331 pg/mL; and
patients with acquired aplastic anemia, 460 pg/mL. Concentrations of flt3
ligand in blood are, therefore, specifically elevated to a level that may
be physiologically relevant in hematopoietic disorders with a suspected
stem cell component. The elevated flt3 ligand concentrations in these
individuals may be part of a compensatory hematopoietic response to boost
the level of progenitor cells.
Volume 86,
Issue 11,
pp. 4091-4096,
12/01/1995
Copyright © 1995 by The American Society of Hematology
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