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Differential expression of integrins on human thymocyte subpopulations
CF Mojcik, DR Salomon, AC Chang and EM Shevach
Cellular Immunology Section, National Institute of Allergy and Infectious
Diseases, National Institutes of Health, Bethesda, MD 20892- 1892, USA.
Integrins represent a candidate group of cell surface receptors that may
control the homing and population of the thymus by T-cell precursors and
the subsequent migration of developing thymocytes through the thymic
architecture. We have used multiparameter flow cytometric methods to
characterize the expression of several members of the integrin family
(alpha 3 beta 1, alpha 4 beta 1, alpha 5 beta 1, alpha 6 beta 1, and alpha
L beta 2) on thymocyte subpopulations and have correlated integrin
expression with other well-defined thymocyte differentiation markers. alpha
4 beta 1 was expressed by all thymocytes, but expression was highest on
CD4-CD8- double-negative (DN) cells, high on CD+CD8+ double-positive (DP)
cells, and lowest on mature single-positive (SP) cells, alpha 3 beta 1,
alpha 5 beta 1, and alpha 6 beta 1 were present on 13%, 63%, and 26% of
thymocytes, respectively, with maximal levels of expression on DN and SP
cells, and low levels of expression on DP cells. Simultaneous analysis of
alpha 4 beta 1, alpha 5 beta 1, and CD3 expression suggested a pathway of
T-cell differentiation in the thymus in which the majority of the DN cells
were alpha 4 beta 1hi alpha 5 beta 1hi, the DP cells alpha 4 beta 1hi alpha
5 beta 1lo/-, and the most mature SP cells were alpha 4 beta 1int. The
stage-specific expression of integrins strongly implies their functional
involvement during T-cell maturation in the thymus.
Volume 86,
Issue 11,
pp. 4206-4217,
12/01/1995
Copyright © 1995 by The American Society of Hematology
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