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Characterization of T cells immortalized by Tax1 of human T-cell leukemia
virus type 1
T Akagi, H Ono and K Shimotohno
Virology Division, National Cancer Center Research Institute, Tokyo, Japan.
Peripheral blood T cells were immortalized in vitro by introduction of the
Tax1 gene of human T-cell leukemia virus type 1 (HTLV-1) with a retroviral
vector and were characterized for transformation-associated markers.
Long-term observation showed that these Tax1-immortalized T cells
eventually exhibited very similar features that were characteristic of
HTLV-1-immortalized T cells, ie, increased expression of egr-1, c-fos,
IL-2R alpha, and Lyn and decreased expression of Lck and cell-surface CD3
antigen. Among these changes, an increase in the expression of Lyn and a
decrease in the expression of Lck and cell- surface CD3 antigen were
observed only in Tax1-immortalized T cells after long-term culture. The
expression level of Tax1 protein did not differ significantly between early
and late passage of cells, and the cellular clonality was found to be the
same by the analysis of the retroviral vector integration site and the
T-cell receptor beta-chain gene rearrangement pattern. These changes in the
expression of Lyn, Lck, and cell-surface CD3 antigen probably resulted from
indirect effects of Tax1 that appeared after extended culture.
Volume 86,
Issue 11,
pp. 4243-4249,
12/01/1995
Copyright © 1995 by The American Society of Hematology
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