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Negative regulation of early T lymphopoiesis by interleukin-3 and
interleukin-1 alpha
F Hirayama and M Ogawa
Department of Medicine, Medical University of South Carolina, Charleston,
USA.
We recently developed a two-step clonal cell culture system for murine
lymphohematopoietic progenitors that are capable of producing myeloid and
B-lymphoid progenies and characterized their cytokine requirements. We
subsequently observed that addition of interleukin-3 (IL-3) or IL-1 alpha
to permissive cytokine combinations in primary culture abrogates the
B-lymphoid potential but not the myeloid potential of the
lymphohematopoietic progenitors. We now describe a similar negative
regulation of the T-cell potential of the lymphohematopoietic progenitors.
Lin- Ly-6A/E+ marrow cells from 5-fluorouracil-treated mice were plated
individually by micromanipulation in methylcellulose culture with steel
factor (SF) and IL-11 for 8 days. The resulting colonies were tested for
myeloid potential by reculturing part of each colony in secondary myeloid
suspension culture. Remainders of individual primary colonies were injected
intravenously into scid mice for determination of T- and B-lymphoid
potentials. Approximately 10% of the progenitors that differentiated along
myeloid lineages in culture reconstituted T- and B-cell compartments in
scid mice. However, when scid mice were injected with colonies pooled from
cultures containing steel factor, IL-11, and either IL-3 or IL-1 alpha,
there was no reconstitution of thymocytes or spleen T cells. These results
suggest negative regulatory roles for IL-3 and IL-1 alpha in the early
stages of T lymphopoiesis.
Volume 86,
Issue 12,
pp. 4527-4531,
12/15/1995
Copyright © 1995 by The American Society of Hematology
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