|
|
 Previous Article | Table of Contents | Next Article 
Primordial germ cells are capable of producing cells of the hematopoietic
system in vitro
IN Rich
Department of Transfusion Medicine, University of Ulm, Germany.
The identity of the cells giving rise to the hematopoietic system in the
mouse embryo are unknown. The results presented here strongly suggest that
hematopoietic cells are derived from a nonhematopoietic cell population
that has been previously thought to give rise to the germ cells. These
cells are called primordial germ cells (PGCs) and can be recognized as
large cells showing blebbing and pseudopodial extrusions on their surface.
They are alkaline phosphatase (AP) positive and possess a stage-specific
embryonic antigen (SSEA-1) on their surface. They represent a small pool of
cells in the extraembryonic mesoderm at the base of the allantois in late
day-6 embryos. Primordial germ cells from 7.5- and 8.5-day visceral yolk
sac and embryo proper form AP+ and SSEA-1+ colonies within 5 days when
grown on an embryonic fibroblast feeder cell layer in the presence of
leukemia inhibitory factor (LIF), stem cell factor (SCF), and interleukin-3
(IL-3). Individual colonies taken from day-5 cultures can be shown to
differentiate into erythroid lineage cells in secondary methyl cellulose
culture and produce secondary and tertiary PGCs in the presence of LIF,
SCF, and IL-3. Cells taken from the region of the allantois and primitive
streak can form colonies on hydrophilic Teflon (DuPont, Wilmington, DE)
foils precoated with collagen and fibronectin. The cells from these
colonies were then shown to form cobblestone areas on irradiated adult bone
marrow stromal layers, indicating that the most primitive in vitro
hematopoietic stem cell, the cobblestone-area forming cell (CAFC), was
present. PGC colonies were grown in methyl cellulose in the presence of
LIF, SCF, and IL-3 for 5 days, and the colonies were removed and passaged 3
times on pretreated extracellular matrix hydrophilic Teflon foils. After
each passage, the cells were assayed for their differentiation capacity and
PGC content. After the last passage, the number of CAFCs was also
determined. It was found that, under these conditions, the PGC population
expanded more than 400- fold and also contained CAFCs. It is postulated
that the PGC represents a totipotent stem cell population capable of
producing a variety of different cell types including cells of the
hematopoietic system.
Volume 86,
Issue 2,
pp. 463-472,
07/15/1995
Copyright © 1995 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
C. Furuta, H. Ema, S.-i. Takayanagi, T. Ogaeri, D. Okamura, Y. Matsui, and H. Nakauchi
Discordant developmental waves of angioblasts and hemangioblasts in the early gastrulating mouse embryo.
Development,
July 1, 2006;
133(14):
2771 - 2779.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Wilpshaar, M. Bhatia, H. H. H. Kanhai, R. Breese, D. K. Heilman, C. S. Johnson, J. H. F. Falkenburg, and E. F. Srour
Engraftment potential of human fetal hematopoietic cells in NOD/SCID mice is not restricted to mitotically quiescent cells
Blood,
June 17, 2002;
100(1):
120 - 127.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A Manaia, V Lemarchandel, M Klaine, I Max-Audit, P Romeo, F Dieterlen-Lievre, and I Godin
Lmo2 and GATA-3 associated expression in intraembryonic hemogenic sites
Development,
January 2, 2000;
127(3):
643 - 653.
[Abstract]
[PDF]
|
|
|
|
|
|
|
F. Zimmermann and I. N. Rich
Mammalian Homeobox B6 Expression Can Be Correlated With Erythropoietin Production Sites and Erythropoiesis During Development, But Not With Hematopoietic or Nonhematopoietic Stem Cell Populations
Blood,
April 15, 1997;
89(8):
2723 - 2735.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
