|
|
 Previous Article | Table of Contents | Next Article 
Stem cell factor retards differentiation of normal human erythroid
progenitor cells while stimulating proliferation
K Muta, SB Krantz, MC Bondurant and CH Dai
Department of Medicine, Department of Veterans Affairs Medical Center,
Nashville, TN, USA.
Stem cell factor (SCF), the ligand for the c-kit tyrosine kinase receptor,
markedly stimulates the accumulation of erythroid progenitor cells in
vitro. We now report that SCF delays erythroid differentiation among the
progeny of individual erythroid progenitors while greatly increasing the
proliferation of these progeny. These effects appear to be independent of
an effect on maintenance of cell viability. Highly purified day-6 erythroid
colony-forming cells (ECFC), consisting mainly of colony-forming
units-erythroid (CFU-E), were generated from human peripheral blood
burst-forming units-erythroid (BFU-E). Addition of SCF to the ECFC in
serum-free liquid culture, together with erythropoietin (EP) and
insulin-like growth factor 1 (IGF-1), resulted in a marked increase in DNA
synthesis, associated with a delayed peak in cellular benzidine positivity
and a delayed incorporation of 59Fe into hemoglobin compared with cultures
without SCF. In the presence of SCF, the number of ECFC was greatly
expanded during this culture period, and total production of
benzidine-positive cells plus hemoglobin synthesis were ultimately
increased. To determine the effect of SCF on individual ECFC, single-cell
cultures were performed in both semisolid and liquid media. These cultures
demonstrated that SCF, in the presence of EP and IGF-1, acted on single
cells and their descendants to delay erythroid differentiation while
substantially stimulating cellular proliferation, without an enhancement of
viability of the initial cells. This was also evident when the effect of
SCF was determined using clones of ECFC derived from single BFU-E. Our
experiments demonstrate that SCF acts on individual day-6 ECFC to retard
erythroid differentiation while simultaneously providing enhanced
proliferation by a process apparently independent of an effect on cell
viability or programmed cell death.
Volume 86,
Issue 2,
pp. 572-580,
07/15/1995
Copyright © 1995 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
A. Elorza, B. Hyde, H. K. Mikkola, S. Collins, and O. S. Shirihai
UCP2 Modulates Cell Proliferation through the MAPK/ERK Pathway during Erythropoiesis and Has No Effect on Heme Biosynthesis
J. Biol. Chem.,
November 7, 2008;
283(45):
30461 - 30470.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. A. Chasis and N. Mohandas
Erythroblastic islands: niches for erythropoiesis
Blood,
August 1, 2008;
112(3):
470 - 478.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Grebien, M. A. Kerenyi, B. Kovacic, T. Kolbe, V. Becker, H. Dolznig, K. Pfeffer, U. Klingmuller, M. Muller, H. Beug, et al.
Stat5 activation enables erythropoiesis in the absence of EpoR and Jak2
Blood,
May 1, 2008;
111(9):
4511 - 4522.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-A. Kang, Y. Zhou, T. L. Weis, H. Liu, J. Ulaszek, N. Satgurunathan, L. Zhou, K. van Besien, J. Crispino, A. Verma, et al.
Osteopontin Regulates Actin Cytoskeleton and Contributes to Cell Proliferation in Primary Erythroblasts
J. Biol. Chem.,
March 14, 2008;
283(11):
6997 - 7006.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. M. Rhodes, P. Kopsombut, M. C. Bondurant, J. O. Price, and M. J. Koury
Adherence to macrophages in erythroblastic islands enhances erythroblast proliferation and increases erythrocyte production by a different mechanism than erythropoietin
Blood,
February 1, 2008;
111(3):
1700 - 1708.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. M. Perry, O. F. Harandi, and R. F. Paulson
BMP4, SCF, and hypoxia cooperatively regulate the expansion of murine stress erythroid progenitors
Blood,
May 15, 2007;
109(10):
4494 - 4502.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Amoyal, E. Prus, and E. Fibach
Vanadate Elevates Fetal Hemoglobin in Human Erythroid Precursors by Inhibiting Cell Maturation
Experimental Biology and Medicine,
May 1, 2007;
232(5):
654 - 661.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Rimmele, O. Kosmider, P. Mayeux, F. Moreau-Gachelin, and C. Guillouf
Spi-1/PU.1 participates in erythroleukemogenesis by inhibiting apoptosis in cooperation with Epo signaling and by blocking erythroid differentiation
Blood,
April 1, 2007;
109(7):
3007 - 3014.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Vegiopoulos, P. Garcia, N. Emambokus, and J. Frampton
Coordination of erythropoiesis by the transcription factor c-Myb
Blood,
June 15, 2006;
107(12):
4703 - 4710.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Subramanian, H. E. Teal, P. H. Correll, and R. F. Paulson
Resistance to Friend Virus-Induced Erythroleukemia in W/Wv Mice Is Caused by a Spleen-Specific Defect Which Results in a Severe Reduction in Target Cells and a Lack of Sf-Stk Expression
J. Virol.,
December 1, 2005;
79(23):
14586 - 14594.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Munugalavadla, L. C. Dore, B. L. Tan, L. Hong, M. Vishnu, M. J. Weiss, and R. Kapur
Repression of c-Kit and Its Downstream Substrates by GATA-1 Inhibits Cell Proliferation during Erythroid Maturation
Mol. Cell. Biol.,
August 1, 2005;
25(15):
6747 - 6759.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Ohene-Abuakwa, K. A. Orfali, C. Marius, and S. E. Ball
Two-phase culture in Diamond Blackfan anemia: localization of erythroid defect
Blood,
January 15, 2005;
105(2):
838 - 846.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. V. Bhanu, T. A. Trice, Y. T. Lee, N. M. Gantt, P. Oneal, J. D. Schwartz, P. Noel, and J. L. Miller
A sustained and pancellular reversal of gamma-globin gene silencing in adult human erythroid precursor cells
Blood,
January 1, 2005;
105(1):
387 - 393.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-M. Paulus, N. Debili, F. Larbret, J. Levin, and W. Vainchenker
Thrombopoietin responsiveness reflects the number of doublings undergone by megakaryocyte progenitors
Blood,
October 15, 2004;
104(8):
2291 - 2298.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Waskow, G. Terszowski, C. Costa, M. Gassmann, and H.-R. Rodewald
Rescue of lethal c-KitW/W mice by erythropoietin
Blood,
September 15, 2004;
104(6):
1688 - 1695.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. van den Akker, T. van Dijk, M. Parren-van Amelsvoort, K. S. Grossmann, U. Schaeper, K. Toney-Earley, S. E. Waltz, B. Lowenberg, and M. von Lindern
Tyrosine kinase receptor RON functions downstream of the erythropoietin receptor to induce expansion of erythroid progenitors
Blood,
June 15, 2004;
103(12):
4457 - 4465.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I.-J. Chung, C. Dai, and S. B. Krantz
Stem cell factor increases the expression of FLIP that inhibits IFNgamma -induced apoptosis in human erythroid progenitor cells
Blood,
February 15, 2003;
101(4):
1324 - 1328.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
U. Wojda, K. R. Leigh, J. M. Njoroge, K. A. Jackson, B. Natarajan, M. Stitely, and J. L. Miller
Fetal hemoglobin modulation during human erythropoiesis: stem cell factor has "late" effects related to the expression pattern of CD117
Blood,
January 15, 2003;
101(2):
492 - 497.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Strife, D. Wisniewski, C. Liu, C. L. Lambek, Z. Darzynkiewicz, R. T. Silver, and B. Clarkson
Direct Evidence That Bcr-Abl Tyrosine Kinase Activity Disrupts Normal Synergistic Interactions Between Kit Ligand and Cytokines in Primary Primitive Progenitor Cells
Mol. Cancer Res.,
January 1, 2003;
1(3):
176 - 185.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
Y.-E. Claessens, D. Bouscary, J.-M. Dupont, F. Picard, J. Melle, S. Gisselbrecht, C. Lacombe, F. Dreyfus, P. Mayeux, and M. Fontenay-Roupie
In vitro proliferation and differentiation of erythroid progenitors from patients with myelodysplastic syndromes: evidence for Fas-dependent apoptosis
Blood,
March 1, 2002;
99(5):
1594 - 1601.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Matsushima, M. Nakashima, K. Oshima, Y. Abe, J. Nishimura, H. Nawata, T. Watanabe, and K. Muta
Receptor binding cancer antigen expressed on SiSo cells, a novel regulator of apoptosis of erythroid progenitor cells
Blood,
July 15, 2001;
98(2):
313 - 321.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. B. van Dijk, E. van den Akker, M. P.-v. Amelsvoort, H. Mano, B. Lowenberg, and M. von Lindern
Stem cell factor induces phosphatidylinositol 3'-kinase-dependent Lyn/Tec/Dok-1 complex formation in hematopoietic cells
Blood,
November 15, 2000;
96(10):
3406 - 3413.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. O. Fortunel, A. Hatzfeld, and J. A. Hatzfeld
Transforming growth factor-beta : pleiotropic role in the regulation of hematopoiesis
Blood,
September 15, 2000;
96(6):
2022 - 2036.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Fujisaki, M. G. Berger, S. Rose-John, and C. J. Eaves
Rapid Differentiation of a Rare Subset of Adult Human Lin-CD34-CD38- Cells Stimulated by Multiple Growth Factors In Vitro
Blood,
September 15, 1999;
94(6):
1926 - 1932.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. von Lindern, W. Zauner, G. Mellitzer, P. Steinlein, G. Fritsch, K. Huber, B. Lowenberg, and H. Beug
The Glucocorticoid Receptor Cooperates With the Erythropoietin Receptor and c-Kit to Enhance and Sustain Proliferation of Erythroid Progenitors In Vitro
Blood,
July 15, 1999;
94(2):
550 - 559.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Haslauer, K. Baltensperger, and H. Porzig
Erythropoietin- and Stem Cell Factor-Induced DNA Synthesis in Normal Human Erythroid Progenitor Cells Requires Activation of Protein Kinase Calpha and Is Strongly Inhibited by Thrombin
Blood,
July 1, 1999;
94(1):
114 - 126.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Tamir, J. Howard, R. R. Higgins, Y.-J. Li, L. Berger, E. Zacksenhaus, M. Reis, and Y. Ben-David
Fli-1, an Ets-Related Transcription Factor, Regulates Erythropoietin-Induced Erythroid Proliferation and Differentiation: Evidence for Direct Transcriptional Repression of the Rb Gene during Differentiation
Mol. Cell. Biol.,
June 1, 1999;
19(6):
4452 - 4464.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Panzenbock, P. Bartunek, M. Y. Mapara, and M. Zenke
Growth and Differentiation of Human Stem Cell Factor/Erythropoietin-Dependent Erythroid Progenitor Cells In Vitro
Blood,
November 15, 1998;
92(10):
3658 - 3668.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Socolovsky, A. E.J. Fallon, and H. F. Lodish
The Prolactin Receptor Rescues EpoR-/- Erythroid Progenitors and Replaces EpoR in a Synergistic Interaction With c-kit
Blood,
September 1, 1998;
92(5):
1491 - 1496.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. C. Gregory, N. Jiang, K. Todokoro, J. Crouse, R. E. Pacifici, and D. M. Wojchowski
Erythropoietin Receptor and STAT5-Specific Pathways Promote SKT6 Cell Hemoglobinization
Blood,
August 15, 1998;
92(4):
1104 - 1118.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Wessely, E.-M. Deiner, K. C. Lim, G. Mellitzer, P. Steinlein, and H. Beug
Mammalian Granulocyte-Macrophage Colony-stimulating Factor Receptor Expressed in Primary Avian Hematopoietic Progenitors: Lineage-specific Regulation of Proliferation and Differentiation
J. Cell Biol.,
May 18, 1998;
141(4):
1041 - 1051.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. O'Prey, N. Leslie, K. Itoh, W. Ostertag, C. Bartholomew, and P. R. Harrison
Both Stroma and Stem Cell Factor Maintain Long-Term Growth of ELM Erythroleukemia Cells, but Only Stroma Prevents Erythroid Differentiation in Response to Erythropoietin and Interleukin-3
Blood,
March 1, 1998;
91(5):
1548 - 1555.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Joneja, H.-C. Chen, D. Seshasayee, A. L. Wrentmore, and D. M. Wojchowski
Mechanisms of Stem Cell Factor and Erythropoietin Proliferative Co-signaling in FDC2-ER Cells
Blood,
November 1, 1997;
90(9):
3533 - 3545.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M.-Y. Zhang, G. A. Clawson, N. F. Olivieri, L. L. Bell, C. G. Begley, and B. A. Miller
Expression of SCL Is Normal in Transfusion-Dependent Diamond-Blackfan Anemia But Other bHLH Proteins Are Deficient
Blood,
September 1, 1997;
90(5):
2068 - 2074.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. C. Broudy
Stem Cell Factor and Hematopoiesis
Blood,
August 15, 1997;
90(4):
1345 - 1364.
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Komatsu, K. Kirito, R. Shimizu, M. Kunitama, M. Yamada, M. Uchida, M. Takatoku, M. Eguchi, and Y. Miura
In Vitro Development of Erythroid and Megakaryocytic Cells From a UT-7 Subline, UT-7/GM
Blood,
June 1, 1997;
89(11):
4021 - 4033.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C.-H. Dai, S. B. Krantz, and S. T. Sawyer
Polycythemia Vera V. Enhanced Proliferation and Phosphorylation Due to Vanadate Are Diminished in Polycythemia Vera Erythroid Progenitor Cells: A Possible Defect of Phosphatase Activity in Polycythemia Vera
Blood,
May 15, 1997;
89(10):
3574 - 3581.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. L. Darley, T. G. Hoy, P. Baines, R. A. Padua, and A. K. Burnett
Mutant N-RAS Induces Erythroid Lineage Dysplasia in Human CD34+ Cells
J. Exp. Med.,
April 7, 1997;
185(7):
1337 - 1348.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Tarr, S. S. Watowich, and G. D. Longmore
Cell Surface Organization of the Erythropoietin Receptor Complex Differs Depending on its Mode of Activation
J. Biol. Chem.,
April 4, 1997;
272(14):
9099 - 9107.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. M. Jacobs-Helber, K. Penta, Z. Sun, A. Lawson, and S. T. Sawyer
Distinct Signaling from Stem Cell Factor and Erythropoietin in HCD57 Cells
J. Biol. Chem.,
March 14, 1997;
272(11):
6850 - 6853.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. von Lindern, M. P.-v. Amelsvoort, T. van Dijk, E. Deiner, E. van den Akker, S. van Emst-de Vries, P. Willems, H. Beug, and B. Lowenberg
Protein Kinase C alpha Controls Erythropoietin Receptor Signaling
J. Biol. Chem.,
October 27, 2000;
275(44):
34719 - 34727.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
