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A conserved TATA-less proximal promoter drives basal transcription from the
urokinase-type plasminogen activator receptor gene
E Soravia, A Grebe, P De Luca, K Helin, TT Suh, JL Degen and F Blasi
Department of Genetics and Microbiology, University of Milano, Italy.
The urokinase-type plasminogen activator receptor (uPAR) focuses at the
cell surface the activation of pro-uPA and, hence, the formation of
plasmin, thus enhancing directional extracellular proteolysis. To
characterize the transcriptional regulatory mechanisms that control
receptor expression, we have cloned an uPAR DNA segment containing upstream
regulatory sequences from both the human and murine genomes. We report that
a proximal promoter, contained within 180 bp from the major transcription
start sites of the human uPAR gene, drives basal transcription. This region
lacks TATA and CAAT boxes and contains relatively GC-rich proximal
sequences. A subregion of this sequence, highly conserved between human and
murine genes, contains most of the promoter activity and is specifically
bound by HeLa nuclear proteins, one of which belongs to the SP1 class.
Volume 86,
Issue 2,
pp. 624-635,
07/15/1995
Copyright © 1995 by The American Society of Hematology
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