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Laminin and fibronectin promote the chemotaxis of human malignant plasma
cell lines
H Shibayama, S Tagawa, H Hattori, R Inoue, S Katagiri and T Kitani
Department of Hematology and Oncology, Osaka University Medical School,
Japan.
We examined chemotaxis of human plasma cells (PCs) in response to
extracellular matrix proteins (ECMs) in the human PC cell lines FR4ds and
OPM-1ds. The FR4ds cells expressed beta 1+, beta 3-, alpha 2-, alpha 3-,
alpha 4+, alpha 5+, alpha 6+, and alpha v+ integrins, whereas the OPM-1ds
cells expressed beta 1+, beta 3-, alpha 2-, alpha 3+, alpha 4+, alpha 5-,
alpha 6+, and alpha v+. Fibronectin (FN) and laminin (LN) promoted the
chemotaxis of the PCs. An inhibitory assay with anti- integrin monoclonal
antibodies (MoAbs) showed that anti-alpha 4 MoAb partially inhibited the
chemotaxis of FR4ds and completely inhibited the chemotaxis of OPM-1ds.
Anti-alpha 5 MoAb alone had no effect on either of these two lines.
Nevertheless, anti-alpha 5 MoAb completely inhibited chemotaxis when it was
added with anti-alpha 4 in FR4ds, demonstrating a novel complementary role
of VLA-5 toward VLA-4 in the chemotaxis induced by FN. LN facilitated
chemotaxis both in OPM-1ds expressing alpha 3 and alpha 6 integrins and in
FR4ds expressing alpha 6 integrin alone. Anti-alpha 6 MoAb completely
inhibited FR4ds chemotaxis, whereas anti-alpha 3 and -alpha 6 MoAb had
synergistic inhibitory effects on the chemotaxis of OPM-1ds. These results
indicated that the distribution of PCs in human tissue are determined by at
least two factors: the concentration of the ECM proteins FN and LN and the
expression of integrins.
Volume 86,
Issue 2,
pp. 719-725,
07/15/1995
Copyright © 1995 by The American Society of Hematology
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