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Benign marrow progenitors are enriched in the CD34+/HLA-DRlo population but
not in the CD34+/CD38lo population in chronic myeloid leukemia: an analysis
using interphase fluorescence in situ hybridization
JA Kirk, JA Reems, BA Roecklein, DR Van Devanter, EM Bryant, J Radich, S Edmands, A Lee and B Torok-Storb
Fred Hutchinson Cancer Research Center, Seattle, WA 98104-2092, USA.
Fluorescence in situ hybridization (FISH) was used to discriminate between
benign and malignant cells in sorted populations of chronic myelogenous
leukemia (CML) marrow. FISH has the advantage of allowing for a cell by
cell analysis of the breakpoint cluster region (BCR) gene rearrangement
immediately after flow sorting in nondividing G0/G1 cells that are
potentially transcriptionally inactive. We initially selected CD34+ cells
with very low expression of the activation antigen CD38 as a candidate
phenotype for an immature and hypothetically more benign cell population,
but found no enrichment for Ph negativity in that subtype. In five CML
samples, 55% +/- 3.3% (mean +/- SE) of CD34+/CD38hi cells had the BCR gene
rearrangement, similar to 57% +/- 3.7% seen in the CD34+/CD38lo population.
In contrast, subsequent experiments (n = 4) determined that the
CD34+/HLA-DRlo population in CML marrow does contain an increased
proportion of benign cells: 15% +/- 1% of the CD34+/DRlo cells were BCR
rearranged, compared with 52% +/- 5.8% of the CD34+/DRhi cells (P = .001).
Our results indicate that benign progenitors in CML are enriched within the
CD34+ cells with low DR antigen expression, but not low CD38 expression.
One possible interpretation of these observations is that low CD38 antigen
expression is not as useful as low HLA-DR expression for isolating immature
cells.
Volume 86,
Issue 2,
pp. 737-743,
07/15/1995
Copyright © 1995 by The American Society of Hematology
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