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Human mast cells derived from fetal liver cells cultured with stem cell
factor express a functional CD51/CD61 (alpha v beta 3) integrin
Y Shimizu, AM Irani, EJ Brown, LK Ashman and LB Schwartz
Department of Internal Medicine, Virginia Commonwealth University,
Richmond, USA.
Human fetal livers contain progenitor cells that become mast cells after 4
weeks of culture with recombinant human stem cell factor. Expression of
cell surface CD29 (beta 1), CD18 (beta 2), CD61 (beta 3), and beta 5
integrins was investigated on such cells by flow cytometry and adhesion
measurements. High surface expression of CD49e, CD51, and CD61 along with
kit was apparent by 4 weeks of culture, whereas expression of each at day 0
was low to undetectable. CD29 and CD49d were detected on cells from day 0
to 4 weeks of culture; CD49b, CD49c, CD49f, CD18, and CD54 expression was
negligible. The fetal liver- derived mast cells spontaneously adhered to
vitronectin. No evidence for degranulation was found during
vitronectin-dependent adhesion. Adhesion occurred in part through the
CD61/CD51 receptor. No evidence for adhesion to vitronectin through CD29
and beta 5 integrins was obtained. Almost all of the vitronectin-adherent
cells expressed CD51, CD61, kit, and tryptase, and exhibited metachromasia
with toluidine blue. Thus, among the fetal liver-derived cells, developing
mast cells were selectively adherent to vitronectin. These mast cells and
the other cell types present also adhere spontaneously to fibronectin and
to laminin, this adhesion being partially inhibited by antibodies against
CD61 and CD29 integrins. In conclusion, human mast cells acquire functional
vitronectin receptors as they develop from fetal liver progenitors under
the influence of rhSCF. This may be important for the recruitment,
localization, and retention of developing mast cells.
Volume 86,
Issue 3,
pp. 930-939,
08/01/1995
Copyright © 1995 by The American Society of Hematology
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