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Murine embryonic yolk sac cells promote in vitro proliferation of bone
marrow high proliferative potential colony-forming cells
MC Yoder, B King, K Hiatt and DA Williams
Herman B Wells Center for Pediatric Research, Department of Pediatrics,
Indiana University School of Medicine, Indianapolis, USA.
To examine the influence of the hematopoietic microenvironment on
hematopoietic cell proliferation and differentiation during the yolk sac
phase of hematopoiesis, we have recently established cell lines from
embryonic yolk sac visceral endoderm (YSE) and mesoderm (YSM). In the
present experiments, we compared in vitro growth of adult murine bone
marrow high proliferative potential colony-forming cells (HPP-CFC) in
coculture with YSE- and YSM-derived or adult bone marrow stromal cell
lines. Whereas both yolk sac-derived and adult stromal cell lines supported
the proliferation of HPP-CFC during coculture, YSE- and YSM- derived cells
stimulated a significant increase in total HPP-CFC compared with adult bone
marrow stromal cell lines. Conditioned media from both YSE- and YSM-derived
cell lines also stimulated the growth of HPP-CFC in vitro, but only in
combination with exogenous recombinant hematopoietic growth factors.
Although multiple hematopoietic growth factor mRNAs were detected in the
yolk sac-derived cells by polymerase chain reaction, only macrophage
colony-stimulating factor (M-CSF) activity was detected in conditioned
media using an enzyme-linked immunosorbent assay. A neutralizing polyclonal
antibody against M-CSF did not diminish the YSE- or YSM-derived cell line
conditioned media promotion of HPP-CFC colony formation. These results
suggest that murine yolk sac-derived cell lines produce a novel soluble
factor(s) that recruits primitive bone marrow hematopoietic cells to grow
in vitro in response to a combination of hematopoietic growth factors.
Volume 86,
Issue 4,
pp. 1322-1330,
08/15/1995
Copyright © 1995 by The American Society of Hematology
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