|
|
 Previous Article | Table of Contents | Next Article 
Characterization and purification of a primitive hematopoietic cell type in
adult mouse marrow capable of lymphomyeloid differentiation in long-term
marrow "switch" cultures
ME Lemieux, VI Rebel, PM Lansdorp and CJ Eaves
Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, Canada.
In this report, we describe a modification of the assay for long-term
culture-initiating cells (LTC-IC) that allows a subset of murine LTC-IC
(designated as LTC-ICML) to express both their myeloid (M) and lymphoid (L)
differentiative potentials in vitro. The modified assay involves culturing
test cells at limiting dilutions on irradiated mouse marrow feeder layers
for an initial 4 weeks under conditions that support myelopoiesis and then
for an additional week under conditions permissive for B-lymphopoiesis. All
of the clonogenic pre-B progenitors (colony-forming unit [CFU] pre-B)
detected in such postswitch LTC appear to be the progeny of uncommitted
cells present in the original cell suspension because exposure of
lymphoid-restricted progenitors to myeloid LTC conditions for > or = 7
days was found to irreversibly terminate CFU-pre-B production and, in
cultures initiated with limiting numbers of input cells (no progenitors of
any type detected in > 70% of cultures 1 week after the switch), the
presence of CFU-pre-B was tightly associated with the presence of myeloid
clonogenic cells, regardless of the purity of the input population.
Limiting dilution analysis of the proportion of negative cultures measured
for different numbers of input cells showed the frequency of LTC-ICML in
normal adult mouse marrow to be 1 per 5 x 10(5) cells with an enrichment of
approximately 500-fold in the Sca-1+ Lin-WGA+ fraction, as was also found
for competitive in vivo repopulating units (CRU) and conventionally defined
LTC-IC. LTC-ICML also exhibited the same resistance to treatment in vivo
with 5-fluorouracil (5-FU) as CRU and LTC-IC, thereby distinguishing these
three populations from the great majority of both in vitro clonogenic cells
and day 12 CFU-S. The ability to quantitate cells with dual lymphoid and
myeloid differentiation potentials in vitro, without the need for their
prior purification, should facilitate studies of totipotent hematopoietic
stem cell regulation.
Volume 86,
Issue 4,
pp. 1339-1347,
08/15/1995
Copyright © 1995 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
S. Yalcin, X. Zhang, J. P. Luciano, S. K. Mungamuri, D. Marinkovic, C. Vercherat, A. Sarkar, M. Grisotto, R. Taneja, and S. Ghaffari
Foxo3 Is Essential for the Regulation of Ataxia Telangiectasia Mutated and Oxidative Stress-mediated Homeostasis of Hematopoietic Stem Cells
J. Biol. Chem.,
September 12, 2008;
283(37):
25692 - 25705.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Furuta, H. Ema, S.-i. Takayanagi, T. Ogaeri, D. Okamura, Y. Matsui, and H. Nakauchi
Discordant developmental waves of angioblasts and hemangioblasts in the early gastrulating mouse embryo.
Development,
July 1, 2006;
133(14):
2771 - 2779.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. K. Richards, F. Liu, H. Iwasaki, K. Akashi, and D. C. Link
Pivotal role of granulocyte colony-stimulating factor in the development of progenitors in the common myeloid pathway
Blood,
November 15, 2003;
102(10):
3562 - 3568.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Carlo-Stella, M. Di Nicola, M. Magni, P. Longoni, M. Milanesi, C. Stucchi, L. Cleris, F. Formelli, and M. A. Gianni
Defibrotide in Combination with Granulocyte Colony-stimulating Factor Significantly Enhances the Mobilization of Primitive and Committed Peripheral Blood Progenitor Cells in Mice
Cancer Res.,
November 1, 2002;
62(21):
6152 - 6157.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Maguer-Satta, R. Oostendorp, D. Reid, and C. J. Eaves
Evidence that ceramide mediates the ability of tumor necrosis factor to modulate primitive human hematopoietic cell fates
Blood,
December 15, 2000;
96(13):
4118 - 4123.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Blazsek, J. Chagraoui, and B. Peault
Ontogenic emergence of the hematon, a morphogenetic stromal unit that supports multipotential hematopoietic progenitors in mouse bone marrow
Blood,
December 1, 2000;
96(12):
3763 - 3771.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. T.-L. Roux, V. Roullot, C. Poujol, T. Kortulewski, P. Nurden, and G. Marguerie
Thrombasthenic mice generated by replacement of the integrin alpha IIb gene: demonstration that transcriptional activation of this megakaryocytic locus precedes lineage commitment
Blood,
August 15, 2000;
96(4):
1399 - 1408.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Lauzurica, D. Sancho, M. Torres, B. Albella, M. Marazuela, T. Merino, J. A. Bueren, C. Martinez-A, and F. Sanchez-Madrid
Phenotypic and functional characteristics of hematopoietic cell lineages in CD69-deficient mice
Blood,
April 1, 2000;
95(7):
2312 - 2320.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Ramsfjell, D. Bryder, H. Bjorgvinsdottir, S. Kornfalt, L. Nilsson, O. J. Borge, and S. E.W. Jacobsen
Distinct Requirements for Optimal Growth and In Vitro Expansion of Human CD34+CD38- Bone Marrow Long-Term Culture-Initiating Cells (LTC-IC), Extended LTC-IC, and Murine In Vivo Long-Term Reconstituting Stem Cells
Blood,
December 15, 1999;
94(12):
4093 - 4102.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. S. Miller, V. McCullar, and C. M. Verfaillie
Ex Vivo Culture of CD34+/Lin-/DR- Cells in Stroma-Derived Soluble Factors, Interleukin-3, and Macrophage Inflammatory Protein-1alpha Maintains Not Only Myeloid But Also Lymphoid Progenitors in a Novel Switch Culture Assay
Blood,
June 15, 1998;
91(12):
4516 - 4522.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. R. Koller, I. Manchel, and A. K. Smith
Quantitative Long-Term Culture-Initiating Cell Assays Require Accessory Cell Depletion That Can Be Achieved by CD34-Enrichment or 5-Fluorouracil Exposure
Blood,
June 1, 1998;
91(11):
4056 - 4064.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. L. Miller and C. J. Eaves
Expansion in vitro of adult murine hematopoietic stem cells with transplantable lympho-myeloid reconstituting ability
PNAS,
December 9, 1997;
94(25):
13648 - 13653.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Conneally, J. Cashman, A. Petzer, and C. Eaves
Expansion in vitro of transplantable human cord blood stem cells demonstrated using a quantitative assay of their lympho-myeloid repopulating activity in nonobese diabetic-scid/scid mice
PNAS,
September 2, 1997;
94(18):
9836 - 9841.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. D. Cashman, T. Lapidot, J. C.Y. Wang, M. Doedens, L. D. Shultz, P. Lansdorp, J. E. Dick, and C. J. Eaves
Kinetic Evidence of the Regeneration of Multilineage Hematopoiesis From Primitive Cells in Normal Human Bone Marrow Transplanted Into Immunodeficient Mice
Blood,
June 15, 1997;
89(12):
4307 - 4316.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. C. Berardi, E. Meffre, F. Pflumio, A. Katz, W. Vainchenker, C. Schiff, and L. Coulombel
Individual CD34+CD38lowCD19-CD10- Progenitor Cells From Human Cord Blood Generate B Lymphocytes and Granulocytes
Blood,
May 15, 1997;
89(10):
3554 - 3564.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. D. Randall and I. L. Weissman
Phenotypic and Functional Changes Induced at the Clonal Level in Hematopoietic Stem Cells After 5-Fluorouracil Treatment
Blood,
May 15, 1997;
89(10):
3596 - 3606.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. W. Zandstra, E. Conneally, A. L. Petzer, J. M. Piret, and C. J. Eaves
Cytokine manipulation of primitive human hematopoietic cell self-renewal
PNAS,
April 29, 1997;
94(9):
4698 - 4703.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. L. Miller, V. I. Rebel, C. D. Helgason, P. M. Lansdorp, and C. J. Eaves
Impaired Steel Factor Responsiveness Differentially Affects the Detection and Long-Term Maintenance of Fetal Liver Hematopoietic Stem Cells In Vivo
Blood,
February 15, 1997;
89(4):
1214 - 1223.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
