|
|
 Previous Article | Table of Contents | Next Article 
Human platelet glycoprotein V: its role in enhancing expression of the
glycoprotein Ib receptor
DC Calverley, M Yagi, SM Stray and GJ Roth
Hematology Section, Seattle Veterans Affairs Medical Center, Seattle, WA
98108, USA.
Platelet adhesion to an injured blood vessel wall is a critical initiating
step in hemostasis mediated by a four member receptor complex (glycoprotein
Ib/V/IX) interacting with plasma von Willebrand factor (vWF). The function
of the GPV subunit within this complex is presently undefined. To study the
role of glycoprotein (GP) V within the GPIb receptor complex, we
transfected the GPV subunit gene into a hematopoietic cell line that
constitutively expresses the other three subunits (human erythroleukemia
[HEL] cells). Using flow cytometry, we found transfected GPV was surface
expressed in HEL cells; this, in turn, led to increased surface expression
of the ligand-binding GPIb alpha and GPIX subunits. Radioligand binding
assays showed that GPV- transfected HEL cells bound more vWF than their
non- or mock- transfected counterparts. We employed confocal microscopy of
GPV- transfected HEL cells to show that GPV colocalizes with GPIb alpha on
the cell surface. These findings suggest that the GPV subunit plays a role
within the GPIb receptor complex by enhancing Ib alpha surface expression.
Volume 86,
Issue 4,
pp. 1361-1367,
08/15/1995
Copyright © 1995 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
X. Mo, N. Lu, A. Padilla, J. A. Lopez, and R. Li
The Transmembrane Domain of Glycoprotein Ibbeta Is Critical to Efficient Expression of Glycoprotein Ib-IX Complex in the Plasma Membrane
J. Biol. Chem.,
August 11, 2006;
281(32):
23050 - 23059.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. L. Kahn, T. G. Diacovo, D. F. Bainton, F. Lanza, J. Trejo, and S. R. Coughlin
Glycoprotein V-Deficient Platelets Have Undiminished Thrombin Responsiveness and Do Not Exhibit a Bernard-Soulier Phenotype
Blood,
December 15, 1999;
94(12):
4112 - 4121.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Ramakrishnan, P. S. Reeves, F. DeGuzman, U. Deshpande, K. Ministri-Madrid, R. B. DuBridge, and D. R. Phillips
Increased thrombin responsiveness in platelets from mice lacking glycoprotein V
PNAS,
November 9, 1999;
96(23):
13336 - 13341.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. S. Bastian, B. A. Kwiatkowski, J. Breininger, S. Danner, and G. Roth
Regulation of the Megakaryocytic Glycoprotein IX Promoter by the Oncogenic Ets Transcription Factor Fli-1
Blood,
April 15, 1999;
93(8):
2637 - 2644.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-f. Dong, S. Gao, and J. A. Lopez
Synthesis, Assembly, and Intracellular Transport of the Platelet Glycoprotein Ib-IX-V Complex
J. Biol. Chem.,
November 20, 1998;
273(47):
31449 - 31454.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Fujita, Y. Hashimoto, S. Russell, B. Zieger, and J. Ware
In Vivo Expression of Murine Platelet Glycoprotein Ibalpha
Blood,
July 15, 1998;
92(2):
488 - 495.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. A. Lopez, R. K. Andrews, V. Afshar-Kharghan, and M. C. Berndt
Bernard-Soulier Syndrome
Blood,
June 15, 1998;
91(12):
4397 - 4418.
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. C. Calverley, T. J. Kavanagh, and G. J. Roth
Human Signaling Protein 14-3-3zeta Interacts With Platelet Glycoprotein Ib Subunits Ibalpha and Ibbeta
Blood,
February 15, 1998;
91(4):
1295 - 1303.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-F. Dong, G. Sae-Tung, and J. A. Lopez
Role of Glycoprotein V in the Formation of the Platelet High-Affinity Thrombin-Binding Site
Blood,
June 15, 1997;
89(12):
4355 - 4363.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. S. Bastian, M. Yagi, C. Chan, and G. J. Roth
Analysis of the Megakaryocyte Glycoprotein IX Promoter Identifies Positive and Negative Regulatory Domains and Functional GATA and Ets Sites
J. Biol. Chem.,
August 2, 1996;
271(31):
18554 - 18560.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
