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This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by el-Farrash, M. Articles by Harada, S Search for Related Content PubMed PubMed Citation Articles by el-Farrash, M. Articles by Harada, S Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Isolation of human T-cell leukemia virus type I from a transformed T- cell line derived spontaneously from lymphocytes of a seronegative Egyptian patient with mycosis fungoides MA el-Farrash, HA Salem, MJ Kuroda, K Morizono, M Kannagi and S Harada Department of Biodefense and Medical Virology, Kumamoto University School of Medicine, Japan. Mycosis fungoides (MF) is a rare form of cutaneous T-cell lymphoma that may be associated with human T-cell leukemia virus type I (HTLV-I) infection. Using the polymerase chain reaction, the HTLV-I pX region was constantly detected in the genomic DNA extracted from peripheral blood mononuclear cells (PBMCs) of an HTLV-I antibody-seronegative Egyptian MF patient enrolled in a study to isolate HTLV-I from North Africa. A CD4+ and interleukin-2 (IL-2) receptor-positive T-cell line was established when the phytohemagglutinin-stimulated PBMCs of that patient were maintained in IL-2-containing culture medium. The cell line (EMF) was initially IL-2 dependent and then became IL-2 independent after gradual withdrawal of the IL-2. The cells reacted positively with monoclonal antibodies specific for the HTLV-I Env or HTLV-I Gag proteins. Using the Southern blot analysis, HTLV-I provirus could be detected in the genomic DNA extracted from the EMF cells. Limited nucleotide sequence of the env region showed more than 95% homology between the EMF provirus and other known HTLV-I isolates. Western blot analysis of the cell lysates showed the expression of the HTLV-I structural proteins. These data imply that a transforming HTLV-I provirus may be present, at least in certain cases of MF, regardless of the presence or absence of the specific antibodies. Volume 86, Issue 5, pp. 1842-1849, 09/01/1995 Copyright © 1995 by The American Society of Hematology CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: Y. Koya, T. Ohashi, H. Kato, S. Hanabuchi, T. Tsukahara, F. Takemura, K.-i. Etoh, M. Matsuoka, M. Fujii, and M. Kannagi Establishment of a Seronegative Human T-Cell Leukemia Virus Type 1 (HTLV-1) Carrier State in Rats Inoculated with a Syngeneic HTLV-1-Immortalized T-Cell Line Preferentially Expressing Tax J. Virol., August 1, 1999; 73(8): 6436 - 6443. [Abstract] [Full Text]

	Copyright © 1995 by American Society of Hematology         Online ISSN: 1528-0020