|
|
 Previous Article | Table of Contents | Next Article 
Granulocyte colony-stimulating factor downregulates allogeneic immune
responses by posttranscriptional inhibition of tumor necrosis factor- alpha
production
A Kitabayashi, M Hirokawa, Y Hatano, M Lee, J Kuroki, H Niitsu and AB Miura
Department of Internal Medicine, Akita University School of Medicine,
Japan.
We report downregulatory effects of granulocyte colony-stimulating factor
(G-CSF) on allogeneic immune responses in vitro. G-CSF did not affect the
proliferative response of peripheral blood mononuclear cells (PBMC) against
allogeneic Daudi cells but did inhibit tumor necrosis factor (TNF)-alpha
secretion. In contrast with G-CSF, granulocyte- macrophage (GM)-CSF and
interleukin (IL)-3 enhanced alloactivation- induced TNF-alpha production.
G-CSF-mediated suppression of TNF-alpha production was not affected by
fixation of stimulators. G-CSF did not inhibit TNF-alpha mRNA expression or
accelerate mRNA degradation, whereas pentoxifylline inhibited the
expression of TNF-alpha mRNA. These results indicate that G-CSF acts
directly on responder cells and modulates TNF-alpha production at
posttranscriptional levels. Suppression of TNF-alpha secretion was
accompanied by an increase of intracellular cyclic adenosine monophosphate
(cAMP) concentration in alloactivated PBMC. The cell-permeable cAMP
analogue, dibutyryl cAMP, suppressed TNF-alpha secretion without affecting
TNF-alpha mRNA expression. G-CSF showed an inhibitory effect on the
development of cytotoxic effector cells against allogeneic Daudi cells.
Anti-TNF-alpha monoclonal antibody (MoAb) also inhibited the induction of
cytolytic activity, and the inhibitory effects of G-CSF and anti-TNF-alpha
MoAb on killer activity generation were overcome by adding exogenous TNF-
alpha. Hence, impaired generation of cytolytic effector cells by G-CSF is
believed to be the result of reduced TNF-alpha production. Collectively,
the results described above suggest that G-CSF downregulates allogeneic
immune responses by posttranscriptionally inhibiting TNF-alpha production.
Volume 86,
Issue 6,
pp. 2220-2227,
09/15/1995
Copyright © 1995 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
A. R. Fraser, G. Cook, I. M. Franklin, J. G. Templeton, M. Campbell, T. L. Holyoake, and J. D. M. Campbell
Immature monocytes from G-CSF-mobilized peripheral blood stem cell collections carry surface-bound IL-10 and have the potential to modulate alloreactivity
J. Leukoc. Biol.,
October 1, 2006;
80(4):
862 - 869.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Rutella, F. Zavala, S. Danese, H. Kared, and G. Leone
Granulocyte Colony-Stimulating Factor: A Novel Mediator of T Cell Tolerance
J. Immunol.,
December 1, 2005;
175(11):
7085 - 7091.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Crough, M. Nieda, and A. J. Nicol
Granulocyte Colony-Stimulating Factor Modulates {alpha}-Galactosylceramide-Responsive Human V{alpha}24+V{beta}11+ NKT Cells
J. Immunol.,
October 15, 2004;
173(8):
4960 - 4966.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Valteau-Couanet, C. Leboulaire, K. Maincent, M. Tournier, O. Hartmann, J. Benard, F. Beaujean, C. Boccaccio, L. Zitvogel, and E. Angevin
Dendritic cells for NK/LAK activation: rationale for multicellular immunotherapy in neuroblastoma patients
Blood,
September 18, 2002;
100(7):
2554 - 2561.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Korbling and P. Anderlini
Peripheral blood stem cell versus bone marrow allotransplantation: does the source of hematopoietic stem cells matter?
Blood,
November 15, 2001;
98(10):
2900 - 2908.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. S. Joshi, J. C. Lynch, S. Z. Pavletic, S. R. Tarantolo, S. J. Pirruccello, A. Kessinger, and M. R. Bishop
Decreased immune functions of blood cells following mobilization with granulocyte colony-stimulating factor: association with donor characteristics
Blood,
September 15, 2001;
98(6):
1963 - 1970.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. G. Wunderink, K. V. Leeper Jr., R. Schein, S. Nelson, B. P. DeBoisblanc, N. Fotheringham, and E. Logan
Filgrastim in Patients With Pneumonia and Severe Sepsis or Septic Shock
Chest,
February 1, 2001;
119(2):
523 - 529.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Pulendran, J. Banchereau, S. Burkeholder, E. Kraus, E. Guinet, C. Chalouni, D. Caron, C. Maliszewski, J. Davoust, J. Fay, et al.
Flt3-Ligand and Granulocyte Colony-Stimulating Factor Mobilize Distinct Human Dendritic Cell Subsets In Vivo
J. Immunol.,
July 1, 2000;
165(1):
566 - 572.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E.-M. Boneberg, L. Hareng, F. Gantner, A. Wendel, and T. Hartung
Human monocytes express functional receptors for granulocyte colony- stimulating factor that mediate suppression of monokines and interferon-gamma
Blood,
January 1, 2000;
95(1):
270 - 276.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Kawabata, M. Hirokawa, A. Kitabayashi, T. Horiuchi, J. Kuroki, and A. B. Miura
Defective Apoptotic Signal Transduction Pathway Downstream of Caspase-3 in Human B-Lymphoma Cells: A Novel Mechanism of Nuclear Apoptosis Resistance
Blood,
November 15, 1999;
94(10):
3523 - 3530.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Przepiorka, T. L. Smith, J. Folloder, I. Khouri, N. T. Ueno, R. Mehra, M. Korbling, Y. O. Huh, S. Giralt, J. Gajewski, et al.
Risk Factors for Acute Graft-Versus-Host Disease After Allogeneic Blood Stem Cell Transplantation
Blood,
August 15, 1999;
94(4):
1465 - 1470.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Engelhardt, H. Bertz, M. Afting, C. F. Waller, and J. Finke
High- Versus Standard-Dose Filgrastim (rhG-CSF) for Mobilization of Peripheral-Blood Progenitor Cells From Allogeneic Donors and CD34+ Immunoselection
J. Clin. Oncol.,
July 1, 1999;
17(7):
2160 - 2160.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Pan, T. Teshima, G. R. Hill, D. Bungard, Y. S. Brinson, V. S. Reddy, K. R. Cooke, and J. L.M. Ferrara
Granulocyte Colony-Stimulating Factor-Mobilized Allogeneic Stem Cell Transplantation Maintains Graft-Versus-Leukemia Effects Through a Perforin-Dependent Pathway While Preventing Graft-Versus-Host Disease
Blood,
June 15, 1999;
93(12):
4071 - 4078.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Weiss, L. L. Moldawer, and E. M. Schneider
Granulocyte Colony-Stimulating Factor to Prevent the Progression of Systemic Nonresponsiveness in Systemic Inflammatory Response Syndrome and Sepsis
Blood,
January 15, 1999;
93(2):
425 - 439.
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Nawa, T. Teshima, K. Sunami, Y. Hiramatsu, T. Yano, K. Shinagawa, E. Omoto, and M. Harada
Responses of Granulocyte Colony-Stimulating Factor-Mobilized Peripheral Blood Mononuclear Cells to Alloantigen Stimulation
Blood,
August 15, 1997;
90(4):
1716 - 1718.
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Iizuka, H. Kaneko, T. Yamada, H. Kimura, Y. Kokai, and J. Fujimoto
Host F1 Mice Pretreated With Granulocyte Colony-Stimulating Factor Accept Parental Bone Marrow Grafts in Hybrid Resistance System
Blood,
February 15, 1997;
89(4):
1446 - 1451.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
