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Recombinant human thrombopoietin (Mpl ligand) enhances proliferation of
erythroid progenitors
M Kobayashi, JH Laver, T Kato, H Miyazaki and M Ogawa
Department of Pediatrics and Medicine, Medical University of South
Carolina, Charleston, USA.
We have studied the effects of recombinant human thrombopoietin (TPO, Mpl
ligand) on human hematopoiesis in vitro. TPO alone did not support
erythroid burst formation but, in the presence of erythropoietin, it
enhanced erythroid burst formation from CD34+ bone marrow and cord blood
cells. The burst-promoting activity (BPA) was stronger under 5% serum than
30% serum conditions. The direct nature of BPA effects was documented by
replating studies of early erythroid bursts. The BPA of TPO was less than
that of interleukin-3 but was comparable with that of
granulocyte/macrophage colony-stimulating factor and steel factor. The
soluble form of Mpl receptor inhibited burst enhancing effects of TPO,
suggesting that the BPA effects of TPO are mediated through the Mpl
receptor. These results further delineate the physiologic roles of TPO and
may aid in the determination of the clinical usages of TPO.
Volume 86,
Issue 7,
pp. 2494-2499,
10/01/1995
Copyright © 1995 by The American Society of Hematology
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