|
|
 Previous Article | Table of Contents | Next Article 
Bone marrow stromal cells induce myeloid and lymphoid development of the
sorted hematopoietic stem cells in vitro
R Okuyama, M Koguma, N Yanai and M Obinata
Department of Cell Biology, Tohoku University, Sendai, Japan.
Regulation of development of hematopoietic stem cells was examined by
culturing Lin- c-Kit+ Sca1+ stem cells sorted from bone marrow (BM) cells
by fluorescence-activated cell sorting on a layer of TBR59, a BM stromal
cell line established from simian virus 40 T-antigen gene transgenic mice.
The sorted stem cells did not show self-renewal, but two waves (at 7 and 13
days) of a cobblestone formation were induced by the stromal cell layer.
The cobblestones were formed by finite cell division (eight divisions on
average) of sorted Lin- c-Kit+ Sca1+ stem cells, and divided cells were
still immature. The c-Kithigh stem cell population was induced to form the
first wave of cobblestone formation committed to myeloid lineage, whereas
c-Kitlow population was induced to form the second wave of this formation
committed to lymphoid lineage. Both cobblestone formations require c-Kit
function, but very late activation antigen-4-vascular cell adhesion
molecule-1 interaction plays different parts in the two lineages.
Volume 86,
Issue 7,
pp. 2590-2597,
10/01/1995
Copyright © 1995 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
R. A. J. Oostendorp, A. J. Medvinsky, N. Kusadasi, N. Nakayama, K. Harvey, C. Orelio, K. Ottersbach, T. Covey, R. E. Ploemacher, C. Saris, et al.
Embryonal subregion-derived stromal cell lines from novel temperature-sensitive SV40 T antigen transgenic mice support hematopoiesis
J. Cell Sci.,
May 15, 2002;
115(10):
2099 - 2108.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Yanai, N. Matsui, T. Furusawa, T. Okubo, and M. Obinata
Sphingosine-1-phosphate and lysophosphatidic acid trigger invasion of primitive hematopoietic cells into stromal cell layers
Blood,
July 1, 2000;
96(1):
139 - 144.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Takahashi, K. Onodera, H. Motohashi, N. Suwabe, N. Hayashi, N. Yanai, Y. Nabesima, and M. Yamamoto
Arrest in Primitive Erythroid Cell Development Caused by Promoter-specific Disruption of the GATA-1 Gene
J. Biol. Chem.,
May 9, 1997;
272(19):
12611 - 12615.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
