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Vascular cell adhesion molecule 1-positive reticular cells express
interleukin-7 and stem cell factor in the bone marrow
PE Funk, RP Stephan and PL Witte
Department of Cell Biology, Loyola University Medical Center, Maywood, IL
60153, USA.
In vitro studies have defined an essential role for stromal cells in
supporting B-cell development, including production of lymphopoietic
cytokines. It has been suggested that stromal cells are equivalent to
adventitial reticular cells in the marrow; however, evidence of reticular
cells producing cytokines has been difficult to obtain. Staining of bone
marrow (BM) sections with antibodies to interleukin-7 (IL-7) showed a
reticular pattern, mimicking that obtained using antibodies to vascular
cell adhesion molecule 1 (VCAM-1), a molecule present on both stromal cells
in vitro and reticular cells. To more closely examine cytokine production
within normal marrow, an immunomagnetic separation scheme was devised to
directly enrich VCAM-1+ stromal cells. Twenty to thirty percent of cells
isolated in the VCAM- 1+ fraction shared characteristics with stromal cells
from long term BM cultures, including cellular morphology and expression of
alkaline phosphatase and alpha actin. These were termed "reticular stromal"
cells. Immunohistochemical staining showed that virtually all of the latter
cells possessed cytoplasmic IL-7 protein, and about half expressed stem
cell factor. In contrast with cultured stromal cells, very few had
detectable macrophage-colony-stimulating factor. These data constitute the
first report of cytokine expression by marrow reticular cells in vivo. The
implications of this data with respect to the existence of stromal cell
subsets and their regulation of lymphopoiesis is discussed.
Volume 86,
Issue 7,
pp. 2661-2671,
10/01/1995
Copyright © 1995 by The American Society of Hematology
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