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Autoantibodies to phosphatidylethanolamine (PE) recognize a kininogen- PE
complex
T Sugi and JA McIntyre
Center for Reproduction and Transplantation Immunology, Methodist Hospital
of Indiana, Indianapolis 46202, USA.
Demonstration of autoimmune antiphospholipid antibodies (aPA) to negatively
charged phospholipids (PL) in an enzyme-linked immunosorbent assay (ELISA)
requires the presence of certain phospholipid-binding plasma proteins, eg,
beta 2-glycoprotein I. We found a requirement for plasma against the
electrically neutral or zwitterionic phospholipid, phosphatidylethanolamine
(PE). Two of these PE-binding plasma proteins were identified as high
molecular weight kininogen (HMWK) and low molecular weight kininogen
(LMWK). We studied anti-PE antibody (aPE) seropositive plasma from 13
patients with SLE and/or recurrent spontaneous abortions by using partially
purified kininogens and kininogen binding proteins from adult bovine serum
isolated by carboxymethyl (CM)-papain affinity chromatography. Eleven of 13
sera recognized a kininogen-PE complex and/or a kininogen-binding protein-
kininogen-PE complex. Some aPE-positive patient sera were shown to
recognize highly purified HMWK and LMWK by ELISA only when the kininogens
were presented on a PE substrate. These aPE sera did not recognize PE,
HMWK, or LMWK when they were presented independently as the sole antigens
on the ELISA plates. Other aPE-positive sera that did not react with
PE-bound HMWK or LMWK reacted with the CM-papain column eluate when it was
bound to PE, which suggests that these aPE recognize factor XI or
prekallikrein, which normally bind to HMWK. The aPE ELISA reactivity of two
patient sera were inhibited by preincubation of the CM-papain column eluate
in the ELISA plate. These data show that most aPE are not specific for PE
but require the presence of certain PL- binding plasma proteins that are
kininogens or proteins in complex with kininogens. Our studies indicate
that aPE bind to different plasma proteins than those implicated in anionic
PL, aPA ELISA reactivity.
Volume 86,
Issue 8,
pp. 3083-3089,
10/15/1995
Copyright © 1995 by The American Society of Hematology
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