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Molecular analysis of cutaneous B- and T-cell lymphomas
A Neri, NS Fracchiolla, E Roscetti, S Garatti, D Trecca, A Boletini, L Perletti, L Baldini, AT Maiolo and E Berti
Laboratorio di Ematologia Sperimentale e Genetica Molecolare, Universita di
Milano, Ospedale Maggiore, IRCCS, Italy.
Among extranodal non-Hodgkin's lymphomas, primary cutaneous lymphomas (CLs)
represent a consistent group of B- and T-cell malignancies. We investigated
the arrangement of Ig and T-cell receptor (TCR) genes, together with the
involvement of several oncogenes and the tumor- suppressor gene p53, in a
panel of primary cutaneous B- and T-cell lymphomas (CBCLs and CTCLs).
Southern blot analysis was performed to detect rearrangements of the Ig,
c-myc, bcl-1, bcl-2, bcl-3, bcl-6, and the NFKB2/lyt-10 genes in 52 cases
of CBCLs and of the TCR, bcl-3, and NFKB2/lyt-10 genes in 38 cases of
CTCLs. tal-1 gene deletions were analyzed in CTCLs by means of polymerase
chain reaction (PCR). p53 gene mutations were assayed using PCR,
single-strand conformation polymorphism analysis, and direct DNA sequencing
in CBCL and CTCL cases. Clonal rearrangements of Ig genes or oncogenes were
found in 25 of the 52 CBCLs. In particular, we detected rearrangements of
the bcl-1 locus (2 cases), the bcl-2 gene (2 cases), the NFKB2/lyt-10 gene
(2 cases), and the bcl-6 gene (1 case); interestingly, 4 of these cases
showed a germline arrangement of the Ig genes. Clonal rearrangements of TCR
genes were detected in 37 of the 38 CTCLs. Rearrangements of the
NFKB2/lyt-10 gene were present in 2 cases and tal-1 gene deletions in 3
CTCL cases; p53 gene mutations were detected in 1 CTCL case. Overall, our
data indicate that (1) clonal rearrangement of Ig genes is frequently
undetectable by means of Southern blot in CBCLs (60%); (2) genetic lesions
are involved in a limited but significant fraction of primary CLs showing a
molecular marker of clonality (13/62; 20%); and (3) rearrangements of the
bcl-1, bcl-2, or bcl-6 loci, associated with specific subsets of nodal
lymphoid neoplasias, are rarely observed in CBCLs. Moreover, our results
suggest that tal-1 gene deletions may play a pathogenetic role in non-acute
T-cell malignancies and that, in the context of lymphoid malignancies, CLs
may represent a favorable target for the possible oncogenic potential of
the NFKB2/lyt-10 gene.
Volume 86,
Issue 8,
pp. 3160-3172,
10/15/1995
Copyright © 1995 by The American Society of Hematology
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