|
|
 Previous Article | Table of Contents | Next Article 
Inhibition of erythro-myeloid differentiation by constitutive expression of
a DNA binding-deficient c-myb mutant: implication for c- myb function
A Sala, T Bellon, P Melotti, C Peschle and B Calabretta
Department of Microbiology and Immunology, Jefferson Cancer Institute,
Thomas Jefferson University, Philadelphia, PA 19107, USA.
The c-myb proto-oncogene encodes a nuclear protein involved in the
regulation of cell proliferation, differentiation, and development. Myb
protein contains a DNA binding and a transactivating domain thought to
mediate its biologic properties. The DNA binding domain consists of three
repeats (R1, R2, and R3), each containing a highly conserved motif of
tryptophan residues. A c-myb mutant (DR1-myb) lacking the last 46 amino
acids of R1 and 23 amino terminal residues of R2, a region homologous to
the ADA-2 yeast transcriptional adaptor, lost DNA binding ability, but
remained able to transactivate the human heat-shock promoter. Transfection
of murine 32D and murine erythroleukemia (MEL) cell lines with DR1-myb
caused inhibition of cellular differentiation induced by granulocyte
colony-stimulating factor (G-CSF) and dimethyl sulfoxide (DMSO),
respectively. A second c-myb mutant (D-ADA2-myb) lacking the first 23 amino
acids of R2, also lost DNA binding and transactivation activity, but did
not inhibit DMSO-induced differentiation of MEL transfected cells. These
findings suggest that deletion of R1 activates a DNA binding-independent
mechanism of c-myb function, which may involve interaction of Myb with
cellular factors.
Volume 86,
Issue 9,
pp. 3404-3412,
11/01/1995
Copyright © 1995 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
E. Grassilli, P. Salomoni, D. Perrotti, C. Franceschi, and B. Calabretta
Resistance to Apoptosis in CTLL-2 Cells Overexpressing B-Myb Is Associated with B-Myb-dependent bcl-2 Induction
Cancer Res.,
May 1, 1999;
59(10):
2451 - 2456.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Z. Ratajczak, D. Perrotti, P. Melotti, M. Powzaniuk, B. Calabretta, K. Onodera, D. A. Kregenow, B. Machalinski, and A. M. Gewirtz
Myb and Ets Proteins Are Candidate Regulators of c-kit Expression in Human Hematopoietic Cells
Blood,
March 15, 1998;
91(6):
1934 - 1946.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Bellon, D. Perrotti, and B. Calabretta
Granulocytic Differentiation of Normal Hematopoietic Precursor Cells Induced by Transcription Factor PU.1 Correlates With Negative Regulation of the c-myb Promoter
Blood,
September 1, 1997;
90(5):
1828 - 1839.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Salomoni, D. Perrotti, R. Martinez, C. Franceschi, and B. Calabretta
Resistance to apoptosis in CTLL-2 cells constitutively expressing c-Myb is associated with induction of BCL-2 expression and Myb-dependent regulation of bcl-2 promoter activity
PNAS,
April 1, 1997;
94(7):
3296 - 3301.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
