|
|
 Previous Article | Table of Contents | Next Article 
Histamine distribution in human basophil secretory granules undergoing
FMLP-stimulated secretion and recovery
AM Dvorak, DW MacGlashan , ES Morgan and LM Lichtenstein
Department of Pathology, Beth Israel Hospital, Boston, MA 02215, USA.
We examined subcellular histamine localizations in purified human basophils
that were stimulated to degranulate with FMLP using an ultrastructural
enzyme-affinity technique. Basophils were collected at early (0, 20
seconds, 1 minute) and late (10 minutes to 6 hours) time points
poststimulation and were prepared for routine ultrastructural and diamine
oxidase-gold (DAO-gold) cytochemical analysis. Histamine was present in
unaltered cytoplasmic secretory granules (30.77 gold particles per square
micrometer; P < .001 compared with background); specificity controls
(histamine absorption, diamine oxidase digestion) abrogated granule
labeling for histamine. Altered granules in stimulated cells were not
significantly labeled for histamine, as compared with background (P = not
significant); unaltered granules in the same cells contained more histamine
than altered granules (P < .05). During recovery times, spanning 10
minutes to 6 hours, granules again appeared to be electron-dense and
contained histamine (33.49/microns2; P = not significant as compared with
unaltered granules in 1-minute FMLP-stimulated cells, and P < .05 as
compared with altered granules in 1-minute FMLP-stimulated samples). Other
structures devoid of histamine in actively secreting cells included
extruded granules and intragranular and extruded Charcot-Leyden crystals.
Recovering basophils displayed morphologic evidence of material and
membrane conservation, granule content condensation, and biosynthesis.
Subcellular histamine-rich sites in actively recovering basophils included
condensing granules and collections of cytoplasmic vesicles in three
locations: beneath the plasma membrane, adjacent to granules, and in the
Golgi region. These studies show that unaltered granules of actively
releasing human basophils, as well as similar granules that are
reconstituted after FMLP-stimulated degranulation, contain histamine, but
that altered granules in stimulated cells undergoing degranulation are
devoid of histamine. Reconstitution of histamine-rich granules is
associated with DAO-gold-positive cytoplasmic vesicles, suggesting
transport of histamine derived from either new synthesis, re-uptake of
released histamine, or both, to reconstituted granules.
Volume 86,
Issue 9,
pp. 3560-3566,
11/01/1995
Copyright © 1995 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
M. Anlauf, M. K.-H. Schafer, T. Schwark, N. von Wurmb-Schwark, V. Brand, B. Sipos, H.-P. Horny, R. Parwaresch, W. Hartschuh, L. E. Eiden, et al.
Vesicular Monoamine Transporter 2 (VMAT2) Expression in Hematopoietic Cells and in Patients with Systemic Mastocytosis
J. Histochem. Cytochem.,
February 1, 2006;
54(2):
201 - 213.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A Dragonetti, M Baldassarre, R Castino, M Demoz, A Luini, R Buccione, and C Isidoro
The lysosomal protease cathepsin D is efficiently sorted to and secreted from regulated secretory compartments in the rat basophilic/mast cell line RBL
J. Cell Sci.,
January 9, 2000;
113(18):
3289 - 3298.
[Abstract]
[PDF]
|
|
|
|
|
|
|
A. M. Dvorak, J. J. Costa, E. S. Morgan, R. A. Monahan-Earley, and S. J. Galli
Diamine Oxidase-Gold Ultrastructural Localization of Histamine in Human Skin Biopsies Containing Mast Cells Stimulated to Degranulate In Vivo by Exposure to Recombinant Human Stem Cell Factor
Blood,
October 15, 1997;
90(8):
2893 - 2900.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Qu-Hong, L. F. Brown, H. F. Dvorak, and A. M. Dvorak
Ultrastructural Immunogold Localization of Osteopontin in Human Gastric Mucosa
J. Histochem. Cytochem.,
January 1, 1997;
45(1):
21 - 34.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
