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Tissue factor mRNA expression in the endothelium of an intact umbilical
vein
MA Courtney, PJ Haidaris, VJ Marder and LA Sporn
Department of Medicine, University of Rochester School of Medicine and
Dentistry, NY, USA.
Tissue factor (TF) mRNA expression was measured by in situ hybridization in
the endothelium of the intact human umbilical vein after infection with
Rickettsia rickettsii. At 4 hours, R rickettsii organisms were clearly
visible within approximately 70% of endothelial cells by immunocytochemical
staining. Quantitation of TF mRNA expression revealed that the level within
endothelial cells of the infected vein was significantly greater (3.7-fold,
P < .0001) than that detected in uninfected endothelial cells. Serial
sections of the umbilical cord vein were processed for in situ
hybridization, and immunocytochemical staining and showed TF expression in
those endothelial cells that contained R rickettsii organisms.
Immunocytochemical staining for TF antigen at 6 hours was negative, but TF
was clearly demonstrated within macrophages and fibroblasts of both control
and infected umbilical cords. These studies demonstrate that the vascular
endothelial cell, ex vivo, can be directly induced to express TF mRNA. This
observation has not heretofore been clearly demonstrated except for in
cultured endothelial cells. Since R rickettsii infection induces thrombotic
vascular occlusions in patients with Rocky Mountain Spotted Fever, the
results imply a potential role for endothelial cell TF in the pathogenesis
of thrombotic disease.
Volume 87,
Issue 1,
pp. 174-179,
01/01/1996
Copyright © 1996 by The American Society of Hematology
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