|
|
 Previous Article | Table of Contents | Next Article 
Myeloid differentiation and retinoblastoma phosphorylation changes in HL-60
cells induced by retinoic acid receptor- and retinoid X receptor- selective
retinoic acid analogs
SC Brooks , S Kazmer, AA Levin and A Yen
Department of Pathology, Cornell University, Ithaca, NY 14853, USA.
The ability of subtypes of retinoic acid receptors (RARs) and retinoid X
receptors (RXRs) singly and in combination to elicit myeloid
differentiation, G1/0-specific growth arrest, and retinoblastoma (RB) tumor
suppressor protein dephosphorylation was determined in the human
myeloblastic leukemia cell line HL-60 using subtype-selective retinoic acid
(RA) analogs. RA analogs that selectively bind only to RARs (Am580 and/or
TTNPB) or to RXRs (Ro 25-6603, SR11237, and/or SR11234) did not elicit the
above-mentioned three cellular responses. In contrast, simultaneous
treatment with both an RAR-selective ligand (Am580 or TTNPB) and an
RXR-selective ligand (Ro 25-6603, SR11237, or SR11234) induced all three
cellular processes. An RAR alpha-selective ligand used with an
RXR-selective ligand generated the same responses as did all-trans RA or
9-cis RA, which affect both families of receptors, suggesting an important
role for RAR alpha among RAR subtypes in eliciting cellular response.
Consistent with this finding, the RAR alpha antagonist, Ro 41-5253, reduced
the level of the cellular responses elicited by treatment with an RAR
alpha-selective ligand plus RXR-selective ligand. The coupling of the shift
of RB to its hypophosphorylated form with G1/0 arrest and differentiation
in response to ligands is consistent with a possible role of RB as a
downstream target or effector of RAR alpha and RXR in combination.
Volume 87,
Issue 1,
pp. 227-237,
01/01/1996
Copyright © 1996 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
M. Shen and A. Yen
c-Cbl Tyrosine Kinase-binding Domain Mutant G306E Abolishes the Interaction of c-Cbl with CD38 and Fails to Promote Retinoic Acid-induced Cell Differentiation and G0 Arrest
J. Biol. Chem.,
September 18, 2009;
284(38):
25664 - 25677.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Shen and A. Yen
c-Cbl Interacts with CD38 and Promotes Retinoic Acid-Induced Differentiation and G0 Arrest of Human Myeloblastic Leukemia Cells
Cancer Res.,
November 1, 2008;
68(21):
8761 - 8769.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Yen, R. Fenning, R. Chandraratna, P. Walker, and S. Varvayanis
A Retinoic Acid Receptor {beta}/{gamma}-Selective Prodrug (tazarotene) Plus a Retinoid X Receptor Ligand Induces Extracellular Signal-Regulated Kinase Activation, Retinoblastoma Hypophosphorylation, G0 Arrest, and Cell Differentiation
Mol. Pharmacol.,
December 1, 2004;
66(6):
1727 - 1737.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Orita, M. Hirose, S. Takahashi, K. Imaida, N. Ito, K. Shudo, H. Ohigashi, A. Murakami, and T. Shirai
Modifying Effects of 1'-Acetoxychavicol Acetate (ACA) and the Novel Synthetic Retinoids Re-80, Am-580 and Am-55P in a Two-Stage Carcinogenesis Model in Female Rats
Toxicol Pathol,
February 1, 2004;
32(2):
250 - 257.
[Abstract]
[PDF]
|
|
|
|
|
|
|
J. Wightman, M. S. Roberson, T. J. Lamkin, S. Varvayanis, and A. Yen
Retinoic Acid-induced Growth Arrest and Differentiation: Retinoic Acid Up-Regulates CD32 (Fc{gamma}RII) Expression, the Ectopic Expression of Which Retards the Cell Cycle
Mol. Cancer Ther.,
May 1, 2002;
1(7):
493 - 506.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Yen, L. Placanica, S. Bloom, and S. Varvayanis
Polyomavirus Small t Antigen Prevents Retinoic Acid-Induced Retinoblastoma Protein Hypophosphorylation and Redirects Retinoic Acid-Induced G0 Arrest and Differentiation to Apoptosis
J. Virol.,
June 1, 2001;
75(11):
5302 - 5314.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
J. M. Miano and B. C. Berk
Retinoids: New Insight Into Smooth Muscle Cell Growth Inhibition
Arterioscler Thromb Vasc Biol,
May 1, 2001;
21(5):
724 - 726.
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Kinoshita, K. Koike, H. H. Mwamtemi, S. Ito, S. Ishida, Y. Nakazawa, Y. Kurokawa, K. Sakashita, T. Higuchi, K. Takeuchi, et al.
Retinoic acid is a negative regulator for the differentiation of cord blood-derived human mast cell progenitors
Blood,
May 1, 2000;
95(9):
2821 - 2828.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Melnick and J. D. Licht
Deconstructing a Disease: RAR{alpha}, Its Fusion Partners, and Their Roles in the Pathogenesis of Acute Promyelocytic Leukemia
Blood,
May 15, 1999;
93(10):
3167 - 3215.
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Giannini, M. I. Dawson, X.-k. Zhang, and C. J. Thiele
Activation of Three Distinct RXR/RAR Heterodimers Induces Growth Arrest and Differentiation of Neuroblastoma Cells
J. Biol. Chem.,
October 17, 1997;
272(42):
26693 - 26701.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
