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Expression of murine CD38 defines a population of long-term reconstituting
hematopoietic stem cells
TD Randall, FE Lund, MC Howard and IL Weissman
Department of Pathology, Stanford Medical School, CA 94305, USA.
Using a monoclonal antibody to murine CD38, we showed that a population of
adult bone marrow cells that expressed the markers Sca-1 and c-kit but
lacked the lineage markers Mac-1, GR-1, B220, IgM, CD3, CD4, CD8 and CD5
could be subdivided by the expression of CD38. We showed that CD38high
c-kit+ Sca-1+, linlow/-cells sorted from adult bone marrow cultured with
interleukin-3 (IL-3), IL-6, and kit-L produced much larger colonies in
liquid culture at a greater frequency than their CD38low/- counterparts. In
addition, we found that CD36low/ - cells contained most of the day-12
colony-forming units-spleen (CFU-S) but were not long-term reconstituting
cells, whereas the population that expressed higher levels of CD38
contained few, but significant, day-12 CFU-S and virtually all the
long-term reconstituting stem cells. Interestingly, the CD38high Sca-1+
c-kit+ linlow/- cells isolated from day-E14.5 fetal liver were also found
to be long-term reconstituting stem cells. This is in striking contrast to
human hematopoietic progenitors in which the most primitive hematopoietic
cells from fetal tissues lack the expression of CD38. Furthermore, because
antibodies to CD38 could functionally replace antibodies to Thy-1.1 in a
stem cell purification procedure, the use of anti-CD38 may be more
generally applicable to the purification of hematopoietic stem cells from
mouse strains that do not express the Thy-1.1 allele.
Volume 87,
Issue 10,
pp. 4057-4067,
05/15/1996
Copyright © 1996 by The American Society of Hematology
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