|
|
 Previous Article | Table of Contents | Next Article 
Functional heterogeneity of the hematopoietic microenvironment: rare
stromal elements maintain long-term repopulating stem cells
J Wineman, K Moore, I Lemischka and C Muller-Sieburg
Medical Biology Institute, La Jolla, CA 92037, USA.
It has been hypothesized that distinct stromal cells from niches within the
microenvironment that selectively regulate stem cell functions. To test
this hypothesis, we derived a panel of matched stromal cell lines from
murine fetal liver. The lines were immortalized with a retroviral vector
encoding a temperature sensitive SV40 T antigen, to provide a snapshot of
potential heterogeneity of the in vivo stroma compartment. All the stromal
cell lines tested, supported the proliferation and differentiation of
myeloid cells in Dexter type bone marrow cultures. Furthermore, RT-PCR
analysis indicates that these lines are similar with respect to the
production of an array of cytokines. However, the stromal cell lines
differed markedly in their ability to maintain in vitro stem cells with in
vivo repopulating capacity. Stem cell levels were measured in the
competitive repopulation assay, following 3 weeks of coculture on
individual stromal cell lines. Three classes of stromal cell lines were
identified: (1) lines that did not support stem cells, (2) lines that
sustained low levels of stem cells that often showed limited persistence in
vivo, and (3) an infrequent line (1 out of 16 lines tested) that maintained
high levels of primitive, long-term repopulating stem cells. This suggests
that stromal cells that can support primitive stem cells are rare in the
hematopoietic microenvironment. Taken together, these data substantiate the
hypothesis that distinct stromal cells interact selectively with stem
cells.
Volume 87,
Issue 10,
pp. 4082-4090,
05/15/1996
Copyright © 1996 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
A. Y. Lai, A. Watanabe, T. O'Brien, and M. Kondo
Pertussis toxin-sensitive G proteins regulate lymphoid lineage specification in multipotent hematopoietic progenitors
Blood,
June 4, 2009;
113(23):
5757 - 5764.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. A. Weinstein, R. M. Gogal Jr, A. Mustafa, M. R. Prater, and S. D. Holladay
Mid-gestation Exposure of C57BL/6 Mice to 2,3,7,8-Tetrachlorodibenzo-p-dioxin Causes Postnatal Morphologic Changes in the Spleen and Liver
Toxicol Pathol,
July 1, 2008;
36(5):
705 - 713.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. A. Phillips, T. I. Brondstetter, C. A. English, H. E. Lee, E. L. Virts, and M. L. Thoman
IL-7 Gene Therapy in Aging Restores Early Thymopoiesis without Reversing Involution
J. Immunol.,
October 15, 2004;
173(8):
4867 - 4874.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Goodrum, C. T. Jordan, S. S. Terhune, K. High, and T. Shenk
Differential outcomes of human cytomegalovirus infection in primitive hematopoietic cell subpopulations
Blood,
August 1, 2004;
104(3):
687 - 695.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Visnjic, Z. Kalajzic, D. W. Rowe, V. Katavic, J. Lorenzo, and H. L. Aguila
Hematopoiesis is severely altered in mice with an induced osteoblast deficiency
Blood,
May 1, 2004;
103(9):
3258 - 3264.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. C. Zhang and H. F. Lodish
Insulin-like growth factor 2 expressed in a novel fetal liver cell population is a growth factor for hematopoietic stem cells
Blood,
April 1, 2004;
103(7):
2513 - 2521.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Sakai, T. Kajiume, H. Inoue, R. Kanno, M. Miyazaki, Y. Ninomiya, and M. Kanno
TCDD Treatment Eliminates the Long-Term Reconstitution Activity of Hematopoietic Stem Cells
Toxicol. Sci.,
March 1, 2003;
72(1):
84 - 91.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. D. Goodrum, C. T. Jordan, K. High, and T. Shenk
Human cytomegalovirus gene expression during infection of primary hematopoietic progenitor cells: A model for latency
PNAS,
December 10, 2002;
99(25):
16255 - 16260.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. A. Hackney, P. Charbord, B. P. Brunk, C. J. Stoeckert, I. R. Lemischka, and K. A. Moore
A molecular profile of a hematopoietic stem cell niche
PNAS,
October 1, 2002;
99(20):
13061 - 13066.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. E. Muller-Sieburg, R. H. Cho, M. Thoman, B. Adkins, and H. B. Sieburg
Deterministic regulation of hematopoietic stem cell self-renewal and differentiation
Blood,
July 30, 2002;
100(4):
1302 - 1309.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. A. J. Oostendorp, A. J. Medvinsky, N. Kusadasi, N. Nakayama, K. Harvey, C. Orelio, K. Ottersbach, T. Covey, R. E. Ploemacher, C. Saris, et al.
Embryonal subregion-derived stromal cell lines from novel temperature-sensitive SV40 T antigen transgenic mice support hematopoiesis
J. Cell Sci.,
May 15, 2002;
115(10):
2099 - 2108.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. A. J. Oostendorp, K. N. Harvey, N. Kusadasi, M. F. T. R. de Bruijn, C. Saris, R. E. Ploemacher, A. L. Medvinsky, and E. A. Dzierzak
Stromal cell lines from mouse aorta-gonads-mesonephros subregions are potent supporters of hematopoietic stem cell activity
Blood,
February 15, 2002;
99(4):
1183 - 1189.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. E. Bolch, P. W. Patton, D. A. Rajon, A. P. Shah, D. W. Jokisch, and B. A. Inglis
Considerations of Marrow Cellularity in 3-Dimensional Dosimetric Models of the Trabecular Skeleton
J. Nucl. Med.,
January 1, 2002;
43(1):
97 - 108.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. S. Kaufman, E. T. Hanson, R. L. Lewis, R. Auerbach, and J. A. Thomson
Hematopoietic colony-forming cells derived from human embryonic stem cells
PNAS,
September 4, 2001;
(2001)
191362598.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Matsuoka, K. Tsuji, H. Hisakawa, M.-j. Xu, Y. Ebihara, T. Ishii, D. Sugiyama, A. Manabe, R. Tanaka, Y. Ikeda, et al.
Generation of definitive hematopoietic stem cells from murine early yolk sac and paraaortic splanchnopleures by aorta-gonad-mesonephros region-derived stromal cells
Blood,
July 1, 2001;
98(1):
6 - 12.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. D. Lewis, G. Almeida-Porada, J. Du, I. R. Lemischka, K. A. Moore, E. D. Zanjani, and C. M. Verfaillie
Umbilical cord blood cells capable of engrafting in primary, secondary, and tertiary xenogeneic hosts are preserved after ex vivo culture in a noncontact system
Blood,
June 1, 2001;
97(11):
3441 - 3449.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. P. Mojica, S. S. Perry, A. E. Searles, K. S. J. Elenitoba-Johnson, L. J. Pierce, A. Wiesmann, W. B. Slayton, and G. J. Spangrude
Phenotypic Distinction and Functional Characterization of Pro-B Cells in Adult Mouse Bone Marrow
J. Immunol.,
March 1, 2001;
166(5):
3042 - 3051.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. K. Sharma, M. C. Nelson, J. E. Brandt, M. Wessman, N. Mahmud, K. P. Weller, and R. Hoffman
Human CD34+ stem cells express the hiwi gene, a human homologue of the Drosophila gene piwi
Blood,
January 15, 2001;
97(2):
426 - 434.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Blazsek, J. Chagraoui, and B. Peault
Ontogenic emergence of the hematon, a morphogenetic stromal unit that supports multipotential hematopoietic progenitors in mouse bone marrow
Blood,
December 1, 2000;
96(12):
3763 - 3771.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C.-C. Shih, M. C.-T. Hu, J. Hu, Y. Weng, P. J. Yazaki, J. Medeiros, and S. J. Forman
A secreted and LIF-mediated stromal cell-derived activity that promotes ex vivo expansion of human hematopoietic stem cells
Blood,
March 15, 2000;
95(6):
1957 - 1966.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C.-C. Shih, M. C.-T. Hu, J. Hu, J. Medeiros, and S. J. Forman
Long-Term Ex Vivo Maintenance and Expansion of Transplantable Human Hematopoietic Stem Cells
Blood,
September 1, 1999;
94(5):
1623 - 1636.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Bennaceur-Griscelli, C. Tourino, B. Izac, W. Vainchenker, and L. Coulombel
Murine Stromal Cells Counteract the Loss of Long-Term Culture-Initiating Cell Potential Induced by Cytokines in CD34+CD38low/neg Human Bone Marrow Cells
Blood,
July 15, 1999;
94(2):
529 - 538.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Kinoshita, T. Sekiguchi, M.-j. Xu, Y. Ito, A. Kamiya, K.-i. Tsuji, T. Nakahata, and A. Miyajima
Hepatic differentiation induced by oncostatin M attenuates fetal liver hematopoiesis
PNAS,
June 22, 1999;
96(13):
7265 - 7270.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. J. Szilvassy, M. J. Bass, G. Van Zant, and B. Grimes
Organ-Selective Homing Defines Engraftment Kinetics of Murine Hematopoietic Stem Cells and Is Compromised by Ex Vivo Expansion
Blood,
March 1, 1999;
93(5):
1557 - 1566.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. S. Miller, V. McCullar, M. Punzel, I. R. Lemischka, and K. A. Moore
Single Adult Human CD34+/Lin-/CD38- Progenitors Give Rise to Natural Killer Cells, B-Lineage Cells, Dendritic Cells, and Myeloid Cells
Blood,
January 1, 1999;
93(1):
96 - 106.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M.-j. Xu, K. Tsuji, T. Ueda, Y.-s. Mukouyama, T. Hara, F.-C. Yang, Y. Ebihara, S. Matsuoka, A. Manabe, A. Kikuchi, et al.
Stimulation of Mouse and Human Primitive Hematopoiesis by Murine Embryonic Aorta-Gonad-Mesonephros-Derived Stromal Cell Lines
Blood,
September 15, 1998;
92(6):
2032 - 2040.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Ohneda, C. Fennie, Z. Zheng, C. Donahue, H. La, R. Villacorta, B. Cairns, and L. A. Lasky
Hematopoietic Stem Cell Maintenance and Differentiation Are Supported by Embryonic Aorta-Gonad-Mesonephros Region-Derived Endothelium
Blood,
August 1, 1998;
92(3):
908 - 919.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Varnum-Finney, L. E. Purton, M. Yu, C. Brashem-Stein, D. Flowers, S. Staats, K. A. Moore, I. Le Roux, R. Mann, G. Gray, et al.
The Notch Ligand, Jagged-1, Influences the Development of Primitive Hematopoietic Precursor Cells
Blood,
June 1, 1998;
91(11):
4084 - 4091.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Q.-L. Hao, E. M. Smogorzewska, L. W. Barsky, and G. M. Crooks
In Vitro Identification of Single CD34+CD38- Cells With Both Lymphoid and Myeloid Potential
Blood,
June 1, 1998;
91(11):
4145 - 4151.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. L. Miller and C. J. Eaves
Expansion in vitro of adult murine hematopoietic stem cells with transplantable lympho-myeloid reconstituting ability
PNAS,
December 9, 1997;
94(25):
13648 - 13653.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. A. Moore, H. Ema, and I. R. Lemischka
In Vitro Maintenance of Highly Purified, Transplantable Hematopoietic Stem Cells
Blood,
June 15, 1997;
89(12):
4337 - 4347.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. A. Moore, B. Pytowski, L. Witte, D. Hicklin, and I. R. Lemischka
Hematopoietic activity of a stromal cell transmembrane protein containing epidermal growth factor-like repeat motifs
PNAS,
April 15, 1997;
94(8):
4011 - 4016.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. C. Yoder and K. Hiatt
Engraftment of Embryonic Hematopoietic Cells in Conditioned Newborn Recipients
Blood,
March 15, 1997;
89(6):
2176 - 2183.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. S. Kaufman, E. T. Hanson, R. L. Lewis, R. Auerbach, and J. A. Thomson
Hematopoietic colony-forming cells derived from human embryonic stem cells
PNAS,
September 11, 2001;
98(19):
10716 - 10721.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. E. Wright, E. P. Bowman, A. J. Wagers, E. C. Butcher, and I. L. Weissman
Hematopoietic Stem Cells Are Uniquely Selective in Their Migratory Response to Chemokines
J. Exp. Med.,
May 6, 2002;
195(9):
1145 - 1154.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
