|
|
 Previous Article | Table of Contents | Next Article 
Early ontogeny of the human marrow from long bones: an immunohistochemical
study of hematopoiesis and its microenvironment [see comments]
P Charbord, M Tavian, L Humeau and B Peault
Laboratoire d'Etude de l'Hematopoiese, Etablissement de Transfusion
Sanguine, Besancon, France.
We examined long bones from 42 human embryos and fetuses whose gestational
ages ranged from 6 to 28 weeks. Bone rudiment sections were stained using a
panel of monoclonal antibodies directed at antigens expressed by
hematopoietic cells, endothelial cells, smooth muscle cells, fibro-blasts,
and stromal cells, to describe the events preceding and accompanying the
onset of hematopoiesis in the diaphyseal region. Five distinct stages were
identified. Stage I (6.6 to 8.5 gestational weeks [gw]) was that of
entirely cartilaginous rudiments: chondrocytes were dilated and capillaries
with CD34+ endothelial cells were observed in the perichondral limb
mesenchyme. At stage II (8.5-9 gw) chondrolysis was actively proceeding;
numerous CD68+ cells were observed, interspersed within the marrow cavity.
Stage III (9 to 10.5 gw) was characterized by the development of the
vascular bed in the absence of detectable hematopoiesis. At mid-diaphysis,
specific structures that we named primary logettes were discernible; they
consisted of small chambers of connective tissue, framed by a loose network
of CD45-negative cells organized around an arteriole and limited from the
surrounding sinus by a clearcut lining of CD34+ endothelial cells flanked
on their abluminal side by alpha SM actin+ myoid cells. Stage IV (10.5-15
gw) was characterized by the onset of hematopoiesis. Hematopoietic cells
were found exclusively in the primary logettes that had considerably
increased in size. Logettes filled with hematopoietic cells were immersed
within large and almost empty vascular sinuses. Logettes were attached by a
short pedicle to connective tissue adjacent to bone/cartilage remaining
formations; this tissue contained very rare hematopoietic cells. Logettes
were few, usually less than 10 per long bone, and found solely in the
diaphyseal area. Most hematopoietic cells found inside logettes were CD15+
myelocytes; rarely seen were glycophorin A+ immature erythroblasts and
CD34+ nonendothelial cells. Hematopoietic cells within the logettes were in
contact with alpha SM actin+ myoid cells and flattened endothelial-like
(although consistently CD34-negative), aligned cells limiting small
capillary lumina. Stage V (16 gw onward) was that of final organization of
the long bones with areas of fully calcified bone and areas of dense
hematopoiesis where logettes were no longer visible. This study shows three
major features of incipient long bone hematopoiesis: 1) absence of CD34+
hematopoietic precursors before the onset of hematopoiesis and extreme
rarity of those in the emerging blood-forming marrow, 2) predominance of
granulopoiesis, and 3) exclusive development in specific structures
organized by vascular cells. This study also suggests that CD68+ cells are
instrumental in the chondrolysis process while vascular cells (endothelial
and myoid cells) may be the critical microenvironment at the onset of
hematopoiesis.
Volume 87,
Issue 10,
pp. 4109-4119,
05/15/1996
Copyright © 1996 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
A. Barcena, M. O. Muench, M. Kapidzic, and S. J. Fisher
A New Role for the Human Placenta as a Hematopoietic Site Throughout Gestation
Reproductive Sciences,
February 1, 2009;
16(2):
178 - 187.
[Abstract]
[PDF]
|
|
|
|
|
|
|
M. O. Muench
Transplantation NK'Oed in the first trimester
Blood,
December 15, 2008;
112(13):
4790 - 4791.
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Weisberg, R. D. Wright, D. W. McMillin, C. Mitsiades, A. Ray, R. Barrett, S. Adamia, R. Stone, I. Galinsky, A. L. Kung, et al.
Stromal-mediated protection of tyrosine kinase inhibitor-treated BCR-ABL-expressing leukemia cells
Mol. Cancer Ther.,
May 1, 2008;
7(5):
1121 - 1129.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Muguruma, T. Yahata, H. Miyatake, T. Sato, T. Uno, J. Itoh, S. Kato, M. Ito, T. Hotta, and K. Ando
Reconstitution of the functional human hematopoietic microenvironment derived from human mesenchymal stem cells in the murine bone marrow compartment
Blood,
March 1, 2006;
107(5):
1878 - 1887.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H.-G. Kopp, S. T. Avecilla, A. T. Hooper, and S. Rafii
The Bone Marrow Vascular Niche: Home of HSC Differentiation and Mobilization
Physiology,
October 1, 2005;
20(5):
349 - 356.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. T. Zambidis, B. Peault, T. S. Park, F. Bunz, and C. I. Civin
Hematopoietic differentiation of human embryonic stem cells progresses through sequential hematoendothelial, primitive, and definitive stages resembling human yolk sac development
Blood,
August 1, 2005;
106(3):
860 - 870.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. P. Monteiro, A. Benjamin, E. S. Costa, M. A. Barcinski, and A. Bonomo
Normal hematopoiesis is maintained by activated bone marrow CD4+ T cells
Blood,
February 15, 2005;
105(4):
1484 - 1491.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Oberlin, M. Tavian, I. Blazsek, and B. Peault
Blood-forming potential of vascular endothelium in the human embryo
Development,
September 1, 2002;
129(17):
4147 - 4157.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Wilpshaar, E. C. Joekes, F. T. H. Lim, G. A. M. Van Leeuwen, P. J. Van den Boogaard, H. H. H. Kanhai, R. Willemze, and J. H. F. Falkenburg
Magnetic resonance imaging of fetal bone marrow for quantitative definition of the human fetal stem cell compartment
Blood,
June 28, 2002;
100(2):
451 - 457.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Jacenko, D. W. Roberts, M. R. Campbell, P. M. McManus, C. J. Gress, and Z. Tao
Linking Hematopoiesis to Endochondral Skeletogenesis through Analysis of Mice Transgenic for Collagen X
Am. J. Pathol.,
June 1, 2002;
160(6):
2019 - 2034.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Campagnoli, I. A. G. Roberts, S. Kumar, P. R. Bennett, I. Bellantuono, and N. M. Fisk
Identification of mesenchymal stem/progenitor cells in human first-trimester fetal blood, liver, and bone marrow
Blood,
October 15, 2001;
98(8):
2396 - 2402.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Campagnoli, N. Fisk, T. Overton, P. Bennett, T. Watts, and I. Roberts
Circulating hematopoietic progenitor cells in first trimester fetal blood
Blood,
March 15, 2000;
95(6):
1967 - 1972.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Cortes, F. Deschaseaux, N. Uchida, M.-C. Labastie, A. M. Friera, D. He, P. Charbord, and B. Peault
HCA, an Immunoglobulin-Like Adhesion Molecule Present on the Earliest Human Hematopoietic Precursor Cells, Is Also Expressed by Stromal Cells in Blood-Forming Tissues
Blood,
February 1, 1999;
93(3):
826 - 837.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M.-C. Labastie, F. Cortes, P.-H. Romeo, C. Dulac, and B. Peault
Molecular Identity of Hematopoietic Precursor Cells Emerging in the Human Embryo
Blood,
November 15, 1998;
92(10):
3624 - 3635.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-F. Wang, Z.-Y. Liu, and J. E. Groopman
The alpha -Chemokine Receptor CXCR4 Is Expressed on the Megakaryocytic Lineage From Progenitor to Platelets and Modulates Migration and Adhesion
Blood,
August 1, 1998;
92(3):
756 - 764.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
