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Differential coupling of CC chemokine receptors to multiple heterotrimeric
G proteins in human interleukin-2-activated natural killer cells
A al-Aoukaty, TJ Schall and AA Maghazachi
Department of Anatomy, University of Oslo, Norway.
Using two different approaches, we have investigated the types of G
proteins coupled to CC chemokine receptors. First, permeabilization of
interleukin-2-activated natural killer (IANK) cells with streptolysin-O and
introduction of anti-G protein antibodies inside these cells resulted in
the following. (1) Anti-G(s), anti-G(o), and anti-G(z) inhibited the
migration of IANK cells in response to macrophage- inflammatory protein-1
alpha (MIP-1 alpha), monocyte chemoattractant protein-1 (MCP-1), or
regulated on activation normal T cell expressed and secreted (RANTES). (2)
Anti-Gi inhibited their migration in response to MCP-1 or RANTES but not in
response to MIP-1 alpha. Second, incubation of IANK cell membranes with
anti-G protein antibodies before incubating with (gamma-35S) GTP or
(gamma-32P) GTP, resulted in the following. (1) Anti-G(s), anti-G(o), or
anti-G(z) inhibited GTP binding and GTPase activity in the presence of
MIP-1 alpha, or RANTES. (2) Anti- G(i) inhibited GTP binding and GTPase
activity in the presence of MCP-1 or RANTES but not in the presence of
MIP-1 alpha. The inhibitory effect of anti-G protein antibodies was
reversed upon incubating these antibodies with their respective synthetic
peptides before addition to IANK cell membranes. These results suggest that
MCP-1 and RANTES receptors are promiscuously coupled to multiple G proteins
in IANK cell membranes and that this coupling is different from MIP-1 alpha
receptors, which seem to be coupled to G(s), G(o), and G(z) but not to
G(i).
Volume 87,
Issue 10,
pp. 4255-4260,
05/15/1996
Copyright © 1996 by The American Society of Hematology
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