|
|
 Previous Article | Table of Contents | Next Article 
Vasoactive intestinal peptide and pituitary adenylate cyclase- activating
polypeptides (PACAP27) and PACAP38) protect CD4+CD8+ thymocytes from
glucocorticoid-induced apoptosis
M Delgado, E Garrido, C Martinez, J Leceta and RP Gomariz
Departamento de Biologia Celular, Facultad de Ciencias Biologicas,
Universidad Complutense, Madrid, Spain.
In the present study, the effects of vasoactive intestinal peptide (VIP)
and the pituitary adenylate cyclase-activating polypeptides, PACAP27 and
PACAP38, in a concentration range from 10(-13) to 10(-6) mol/L were studied
in vitro on the spontaneous and dexamethasone (DEX)- induced apoptosis in
rat thymocytes. The results show that VIP and both PACAPs inhibit
significantly and in a similar way the DNA fragmentation characteristic of
glucocorticoid-induced apoptosis and increase the cell survival of
thymocytes, with a maximal effect observed at 10(-8) to 10(-9) mol/L. This
study showed the ability of the VIP-receptor (VIP- R) antagonist
[N-Ac-Tyr1,D-Phe2]-GRF(1-29) amide to partially reverse the inhibitory
effect of VIP and both PACAPs on DEX-induced apoptosis, providing evidence
for a specific VIP1-R-mediated response and supporting the involvement of a
single receptor for the three neuropeptides. Phenotypic analysis showed
that VIP, PACAP27, and PACAP38 protect predominantly CD4+CD8+ thymocytes
from glucocorticoid- induced apoptosis. These findings suggest that these
neuropeptides could be involved in intrathymic T-cell maturation.
Volume 87,
Issue 12,
pp. 5152-5161,
06/15/1996
Copyright © 1996 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
D. Vaudry, A. Falluel-Morel, S. Bourgault, M. Basille, D. Burel, O. Wurtz, A. Fournier, B. K. C. Chow, H. Hashimoto, L. Galas, et al.
Pituitary Adenylate Cyclase-Activating Polypeptide and Its Receptors: 20 Years after the Discovery
Pharmacol. Rev.,
September 1, 2009;
61(3):
283 - 357.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. S. R. Sastry, A. J. Smith, Y. Karpova, S. R. Datta, and G. Kulik
Diverse Antiapoptotic Signaling Pathways Activated by Vasoactive Intestinal Polypeptide, Epidermal Growth Factor, and Phosphatidylinositol 3-Kinase in Prostate Cancer Cells Converge on BAD
J. Biol. Chem.,
July 28, 2006;
281(30):
20891 - 20901.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. J. Drucker
Glucagon-Like Peptides: Regulators of Cell Proliferation, Differentiation, and Apoptosis
Mol. Endocrinol.,
February 1, 2003;
17(2):
161 - 171.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Li, T. Hansotia, B. Yusta, F. Ris, P. A. Halban, and D. J. Drucker
Glucagon-like Peptide-1 Receptor Signaling Modulates beta Cell Apoptosis
J. Biol. Chem.,
January 3, 2003;
278(1):
471 - 478.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. J. Middlebrook, C. Martina, Y. Chang, R. J. Lukas, and D. DeLuca
Effects of Nicotine Exposure on T Cell Development in Fetal Thymus Organ Culture: Arrest of T Cell Maturation
J. Immunol.,
September 15, 2002;
169(6):
2915 - 2924.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Yusta, J. Estall, and D. J. Drucker
Glucagon-like Peptide-2 Receptor Activation Engages Bad and Glycogen Synthase Kinase-3 in a Protein Kinase A-dependent Manner and Prevents Apoptosis following Inhibition of Phosphatidylinositol 3-Kinase
J. Biol. Chem.,
July 5, 2002;
277(28):
24896 - 24906.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. M. Erhardt, E. A. Fradinger, L. A. Cervini, J. E. Rivier, and N. M. Sherwood
Early Expression of Pituitary Adenylate Cyclase-Activating Polypeptide and Activation of its Receptor in Chick Neuroblasts
Endocrinology,
April 1, 2001;
142(4):
1616 - 1625.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
R. Ramesh, W.J. Kuenzel, and J.A. Proudman
Increased Proliferative Activity and Programmed Cellular Death in the Turkey Hen Pituitary Gland Following Interruption of Incubation Behavior
Biol Reprod,
February 1, 2001;
64(2):
611 - 618.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
D. Vaudry, B. J. Gonzalez, M. Basille, L. Yon, A. Fournier, and H. Vaudry
Pituitary Adenylate Cyclase-Activating Polypeptide and Its Receptors: From Structure to Functions
Pharmacol. Rev.,
June 1, 2000;
52(2):
269 - 324.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-C. Marie, A. Wakkach, A.-M. Coudray, E. Chastre, S. Berrih-Aknin, and C. Gespach
Functional Expression of Receptors for Calcitonin Gene-Related Peptide, Calcitonin, and Vasoactive Intestinal Peptide in the Human Thymus and Thymomas from Myasthenia Gravis Patients
J. Immunol.,
February 15, 1999;
162(4):
2103 - 2112.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
