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CD34+CD38dim cells in the human thymus can differentiate into T, natural
killer, and dendritic cells but are distinct from pluripotent stem cells
P Res, E Martinez-Caceres, A Cristina Jaleco, F Staal, E Noteboom, K Weijer and H Spits
Division of Immunology, The Netherlands Cancer Institute, Antoni Antoni Van
Leeuwenhoek Huis, Amsterdam, The Netherlands.
Recently we reported that the human thymus contains a minute population of
CD34+CD38dim cells that do not express the T-cell lineage markers CD2 and
CD5. The phenotype of this population resembled that of CD34+CD38dim cells
present in fetal liver, umbilical cord blood, and bone marrow known to be
highly enriched for pluripotent hematopoietic stem cells. In this report we
tested the hypothesis that the CD34+CD38dim thymocytes constitute the most
primitive hematopoietic cells in the thymus using a combination of
phenotypic and functional analyses. It was found that in contrast to
CD34+CD38dim cells from fetal liver and bone marrow, CD34+CD38dim cells
from the thymus express high levels of CD45RA and are negative for Thy-1.
These data indicate that the CD34+CD38dim thymocytes are distinct from
pluripotent stem cells. CD34+CD38dim thymocytes differentiate into T cells
when cocultured with mouse fetal thymic organs. In addition, individual
cells in this population can differentiate either to natural killer cells
in the presence of stem cell factor (SCF), interleukin-7 (IL-7), and IL-2
or to dendritic cells in the presence of SCF, granulocyte- macrophage
colony-stimulating factor, and tumor necrosis factor alpha(TNFalpha),
indicating that CD34+CD38dim thymocytes contain multi- potential
hematopoietic progenitors. To establish which CD34+ fetal liver
subpopulation contains the cells that migrate to the thymus, we
investigated the T-cell-developing potential of CD34+CD38dim and CD34+CD38+
fetal liver cells and found that the capacity of CD34+ fetal liver cells to
differentiate into T cells is restricted to those cells that are CD38dim.
Collectively, these findings indicate that cells from the CD34+CD38dim
fetal liver cell population migrate to the thymus before upregulating CD38
and ommitting to the T-cell lineage.
Volume 87,
Issue 12,
pp. 5196-5206,
06/15/1996
Copyright © 1996 by The American Society of Hematology
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