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Thrombopoietin induces tyrosine phosphorylation of Stat3 and Stat5 in human
blood platelets
Y Miyakawa, A Oda, BJ Druker, H Miyazaki, M Handa, H Ohashi and Y Ikeda
Department of Internal Medicine, Keio University, Tokyo, Japan.
Thrombopoietin is known to be essential for megakaryocytopoiesis and
thrombopoiesis. Recently, we and others have shown that thrombopoietin
induces rapid tyrosine phosphorylation of Jak2 and other proteins in human
platelets and BaF3 cells, genetically engineered to express c- Mpl, a
receptor for thrombopoietin. The Jak family of tyrosine kinases are known
to mediate some of the effects of cytokines or hematopoietic growth factors
by recruitment and tyrosine phosphorylation of a variety of Stat (signal
transducers and activators of transcription) proteins. Hence, we have
investigated whether Stat proteins are present in platelets and, if so,
whether they become tyrosine phosphorylated in response to thrombopoietin.
We immunologically identified Stat1, Stat2, Stat3, and Stat5 in human
platelet lysates. Thrombopoietin induced tyrosine phosphorylation of Stat3
and Stat5 in these cells. Thrombopoietin also induced tyrosine
phosphorylation of Stat3 and Stat5 in FDCP-2 cells genetically engineered
to constitutively express human c-Mpl. Thus, our data indicate that Stat3
and Stat5 may be involved in signal transduction after ligand binding to
c-Mpl and that this event may have a role in
megakaryopoiesis/thrombopoiesis or possibly a mature platelet function such
as aggregation.
Volume 87,
Issue 2,
pp. 439-446,
01/15/1996
Copyright © 1996 by The American Society of Hematology
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October 1, 2008;
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|
|
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|
|
|
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12(22):
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|
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|
|
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|
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F. Campus, P. Lova, A. Bertoni, F. Sinigaglia, C. Balduini, and M. Torti
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July 1, 2005;
280(26):
24386 - 24395.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Kirito, M. Osawa, H. Morita, R. Shimizu, M. Yamamoto, A. Oda, H. Fujita, M. Tanaka, K. Nakajima, Y. Miura, et al.
A functional role of Stat3 in in vivo megakaryopoiesis
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May 1, 2002;
99(9):
3220 - 3227.
[Abstract]
[Full Text]
[PDF]
|
|
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|
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A. Oda, H. Wakao, and H. Fujita
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99(5):
1850 - 1852.
[Abstract]
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|
|
|
|
|
|
|
M.-D. Filippi, F. Porteu, F. L. Pesteur, V. Schiavon, G. A. Millot, W. Vainchenker, F. J. de Sauvage, A. Dubart Kupperschmitt, and F. Sainteny
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99(4):
1174 - 1182.
[Abstract]
[Full Text]
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|
|
|
|
|
|
|
M. Benekli, Z. Xia, K. A. Donohue, L. A. Ford, L. A. Pixley, M. R. Baer, H. Baumann, and M. Wetzler
Constitutive activity of signal transducer and activator of transcription 3 protein in acute myeloid leukemia blasts is associated with short disease-free survival
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January 1, 2002;
99(1):
252 - 257.
[Abstract]
[Full Text]
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|
|
|
|
|
|
|
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STAT5 Induces Macrophage Differentiation of M1 Leukemia Cells Through Activation of IL-6 Production Mediated by NF-{kappa}B p65
J. Immunol.,
October 1, 2001;
167(7):
3652 - 3660.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Gaur, G. J. Murphy, F. J. deSauvage, and A. D. Leavitt
Characterization of Mpl mutants using primary megakaryocyte-lineage cells from mpl{-}/{-} mice: a new system for Mpl structure-function studies
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March 15, 2001;
97(6):
1653 - 1661.
[Abstract]
[Full Text]
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|
|
|
|
|
|
|
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96(13):
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|
|
|
|
|
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96(6):
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|
|
|
|
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95(8):
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|
|
|
|
|
|
|
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[Abstract]
[Full Text]
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|
|
|
|
|
|
|
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95(5):
1663 - 1670.
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|
|
|
|
|
|
|
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94(10):
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|
|
|
|
|
|
|
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|
|
|
|
|
|
|
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August 15, 1999;
94(4):
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|
|
|
|
|
|
|
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|
|
|
|
|
|
|
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April 1, 1999;
93(7):
2369 - 2379.
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[Full Text]
[PDF]
|
|
|
|
|
|
|
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Blood,
January 1, 1999;
93(1):
15 - 24.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Ozaki, A. Oda, H. Wakao, J. Rhodes, B. J. Druker, A. Ishida, M. Wakui, S. Okamoto, K. Morita, M. Handa, et al.
Thrombopoietin Induces Association of Crkl With STAT5 But Not STAT3 in Human Platelets
Blood,
December 15, 1998;
92(12):
4652 - 4662.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Okajima, I. Matsumura, T. Nishiura, K. Hashimoto, H. Yoshida, J. Ishikawa, H. Wakao, A. Yoshimura, Y. Kanakura, Y. Tomiyama, et al.
Insulin-like Growth Factor-I Augments Erythropoietin-induced Proliferation through Enhanced Tyrosine Phosphorylation of STAT5
J. Biol. Chem.,
September 4, 1998;
273(36):
22877 - 22883.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Oda, K. Sawada, B. J. Druker, K. Ozaki, H. Takano, K. Koizumi, Y. Fukada, M. Handa, T. Koike, and Y. Ikeda
Erythropoietin Induces Tyrosine Phosphorylation of Jak2, STAT5A, and STAT5B in Primary Cultured Human Erythroid Precursors
Blood,
July 15, 1998;
92(2):
443 - 451.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. R. Moliterno, W. D. Hankins, and J. L. Spivak
Impaired Expression of the Thrombopoietin Receptor by Platelets from Patients with Polycythemia Vera
N. Engl. J. Med.,
February 26, 1998;
338(9):
572 - 580.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Hong, D. Dumenil, B. van der Loo, F. Goncalves, W. Vainchenker, and J. D. Erusalimsky
Protein Kinase C Mediates the Mitogenic Action of Thrombopoietin in c-Mpl-Expressing UT-7 Cells
Blood,
February 1, 1998;
91(3):
813 - 822.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Horikawa, I. Matsumura, K. Hashimoto, M. Shiraga, S. Kosugi, S. Tadokoro, T. Kato, H. Miyazaki, Y. Tomiyama, Y. Kurata, et al.
Markedly Reduced Expression of Platelet c-mpl Receptor in Essential Thrombocythemia
Blood,
November 15, 1997;
90(10):
4031 - 4038.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Ballmaier, H. Schulze, K. Cherkaoui, N. Wittner, S. Lynen, S. Wolters, J. Bogenberger, and K. Welte
Thrombopoietin in Patients With Congenital Thrombocytopenia and Absent Radii: Elevated Serum Levels, Normal Receptor Expression, But Defective Reactivity to Thrombopoietin
Blood,
July 15, 1997;
90(2):
612 - 619.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Goncalves, C. Lacout, J.-L. Villeval, F. Wendling, W. Vainchenker, and D. Dumenil
Thrombopoietin Does Not Induce Lineage-Restricted Commitment of Mpl-R Expressing Pluripotent Progenitors But Permits Their Complete Erythroid and Megakaryocytic Differentiation
Blood,
May 15, 1997;
89(10):
3544 - 3553.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Matsumoto, M. Masuhara, K. Mitsui, M. Yokouchi, M. Ohtsubo, H. Misawa, A. Miyajima, and A. Yoshimura
CIS, a Cytokine Inducible SH2 Protein, Is a Target of the JAK-STAT5 Pathway and Modulates STAT5 Activation
Blood,
May 1, 1997;
89(9):
3148 - 3154.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Kato, A. Oda, Y. Inagaki, H. Ohashi, A. Matsumoto, K. Ozaki, Y. Miyakawa, H. Watarai, K. Fuju, A. Kokubo, et al.
Thrombin cleaves recombinant human thrombopoietin: One of the proteolytic events that generates truncated forms of thrombopoietin
PNAS,
April 29, 1997;
94(9):
4669 - 4674.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. J. Fielder, P. Hass, M. Nagel, E. Stefanich, R. Widmer, G. L. Bennett, G.-A. Keller, F. J. de Sauvage, and D. Eaton
Human Platelets as a Model for the Binding and Degradation of Thrombopoietin
Blood,
April 15, 1997;
89(8):
2782 - 2788.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Miyakawa, A. Oda, B. J. Druker, K. Ozaki, M. Handa, H. Ohashi, and Y. Ikeda
Thrombopoietin and Thrombin Induce Tyrosine Phosphorylation of Vav in Human Blood Platelets
Blood,
April 15, 1997;
89(8):
2789 - 2798.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Iwatsuki, T. Endo, H. Misawa, M. Yokouchi, A. Matsumoto, M. Ohtsubo, K. J. Mori, and A. Yoshimura
STAT5 Activation Correlates with Erythropoietin Receptor-mediated Erythroid Differentiation of an Erythroleukemia Cell Line
J. Biol. Chem.,
March 28, 1997;
272(13):
8149 - 8152.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. G. Drachman and K. Kaushansky
Dissecting the thrombopoietin receptor: Functional elements of the Mpl cytoplasmic domain
PNAS,
March 18, 1997;
94(6):
2350 - 2355.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. C. Broudy, N. L. Lin, D. F. Sabath, T. Papayannopoulou, and K. Kaushansky
Human Platelets Display High-Affinity Receptors for Thrombopoietin
Blood,
March 15, 1997;
89(6):
1896 - 1904.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. G. Drachman, D. F. Sabath, N. E. Fox, and K. Kaushansky
Thrombopoietin Signal Transduction in Purified Murine Megakaryocytes
Blood,
January 15, 1997;
89(2):
483 - 492.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Kroner, K. Eybrechts, and J.-W. N. Akkerman
Dual Regulation of Platelet Protein Kinase B
J. Biol. Chem.,
September 1, 2000;
275(36):
27790 - 27798.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Miyakawa, P. Rojnuckarin, T. Habib, and K. Kaushansky
Thrombopoietin Induces Phosphoinositol 3-Kinase Activation through SHP2, Gab, and Insulin Receptor Substrate Proteins in BAF3 Cells and Primary Murine Megakaryocytes
J. Biol. Chem.,
January 19, 2001;
276(4):
2494 - 2502.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Miyakawa, J. G. Drachman, B. Gallis, A. Kaushansky, and K. Kaushansky
A Structure-Function Analysis of Serine/Threonine Phosphorylation of the Thrombopoietin Receptor, c-Mpl
J. Biol. Chem.,
October 6, 2000;
275(41):
32214 - 32219.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
