|
|
 Previous Article | Table of Contents | Next Article 
Hematopoietic defects in mice lacking the sialomucin CD34
J Cheng, S Baumhueter, G Cacalano, K Carver-Moore, H Thibodeaux, R Thomas, HE Broxmeyer, S Cooper, N Hague, M Moore and LA Lasky
Department of Molecular Oncology, Genentech, Inc, South San Francisco, CA,
94108, USA.
Although the pluripotent hematopoietic stem cell can only be definitively
identified by its ability to reconstitute the various mature blood
lineages, a diversity of cell surface antigens have also been specifically
recognized on this subset of hematopoietic progenitors. One such stem
cell-associated antigen is the sialomucin CD34, a highly O-glycosylated
cell surface glycoprotein that has also been shown to be expressed on all
vascular endothelial cells throughout murine embryogenesis as well as in
the adult. The functional significance of CD34 expression on hematopoietic
progenitor cells and developing blood vessels is unknown. To analyze the
involvement of CD34 in hematopoiesis, we have produced both embryonic stem
(ES) cells and mice that are null for the expression of this mucin.
Analysis of yolk saclike hematopoietic development in embryoid bodies
derived from CD34- null ES cells showed a significant delay in both
erythroid and myeloid differentiation that could be reversed by
transfection of the mutant ES cells with CD34 constructs expressing either
a complete or truncated cytoplasmic domain. Measurements of colony-forming
activity of hematopoietic progenitor cells derived from yolk sacs or fetal
livers isolated from CD34-null embryos also showed a decreased number of
these precursor cells. In spite of these diminished embryonic hematopoietic
progenitor numbers, the CD34-null mice developed normally, and the
hematopoietic profile of adult blood appeared typical. However, the
colony-forming activity of hematopoietic progenitors derived from both bone
marrow and spleen is significantly reduced in adult CD34-deficient animals,
and these CD34-deficient progenitors also appear to be unable to expand in
liquid cultures in response to hematopoietic growth factors. Even with
these apparent progenitor cell deficiencies, CD34- null animals showed
kinetics of erythroid, myeloid, and platelet recovery after sublethal
irradiation that are indistinguishable from wild-type mice. These data
strongly suggest that CD34 plays an important role in the formation of
progenitor cells during both embryonic and adult hematopoiesis. However,
the hematopoietic sites of adult CD34-deficient mice may still have a
significant reservoir of progenitor cells that allows for normal recovery
after nonmyeloablative peripheral cell depletion.
Volume 87,
Issue 2,
pp. 479-490,
01/15/1996
Copyright © 1996 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
J. E. Testa, A. Chrastina, P. Oh, Y. Li, H. Witkiewicz, M. Czarny, T. Buss, and J. E. Schnitzer
Immunotargeting and cloning of two CD34 variants exhibiting restricted expression in adult rat endothelia in vivo
Am J Physiol Lung Cell Mol Physiol,
August 1, 2009;
297(2):
L251 - L262.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. S. Nielsen and K. M. McNagny
Novel functions of the CD34 family
J. Cell Sci.,
November 15, 2008;
121(22):
3683 - 3692.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M.-R. Blanchet, S. Maltby, D. J. Haddon, H. Merkens, L. Zbytnuik, and K. M. McNagny
CD34 facilitates the development of allergic asthma
Blood,
September 15, 2007;
110(6):
2005 - 2012.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. E. Broxmeyer, S. Sehra, S. Cooper, L. M. Toney, S. Kusam, J. J. Aloor, C. C. Marchal, M. C. Dinauer, and A. L. Dent
Aberrant Regulation of Hematopoiesis by T Cells in BAZF-Deficient Mice
Mol. Cell. Biol.,
August 1, 2007;
27(15):
5275 - 5285.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Ito, C. R. Mantel, M.-K. Han, S. Basu, S. Fukuda, S. Cooper, and H. E. Broxmeyer
Mad2 is required for optimal hematopoiesis: Mad2 associates with c-Kit in MO7e cells
Blood,
March 1, 2007;
109(5):
1923 - 1930.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Shi and D. J. Garry
Muscle stem cells in development, regeneration, and disease.
Genes & Dev.,
July 1, 2006;
20(13):
1692 - 1708.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Hernandez-Gonzalez, I. Gonzalez-Robayna, M. Shimada, C. M. Wayne, S. A. Ochsner, L. White, and J. S. Richards
Gene Expression Profiles of Cumulus Cell Oocyte Complexes during Ovulation Reveal Cumulus Cells Express Neuronal and Immune-Related Genes: Does this Expand Their Role in the Ovulation Process?
Mol. Endocrinol.,
June 1, 2006;
20(6):
1300 - 1321.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. A. Denburg and S. F. van Eeden
Bone marrow progenitors in inflammation and repair: new vistas in respiratory biology and pathophysiology.
Eur. Respir. J.,
March 1, 2006;
27(3):
441 - 445.
[Full Text]
[PDF]
|
|
|
|
|
|
|
U. Fiedler, S. Christian, S. Koidl, D. Kerjaschki, M. S. Emmett, D. O. Bates, G. Christofori, and H. G. Augustin
The Sialomucin CD34 Is a Marker of Lymphatic Endothelial Cells in Human Tumors
Am. J. Pathol.,
March 1, 2006;
168(3):
1045 - 1053.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Doyonnas, J. S. Nielsen, S. Chelliah, E. Drew, T. Hara, A. Miyajima, and K. M. McNagny
Podocalyxin is a CD34-related marker of murine hematopoietic stem cells and embryonic erythroid cells
Blood,
June 1, 2005;
105(11):
4170 - 4178.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. M. Lemoli, D. Ferrari, M. Fogli, L. Rossi, C. Pizzirani, S. Forchap, P. Chiozzi, D. Vaselli, F. Bertolini, T. Foutz, et al.
Extracellular nucleotides are potent stimulators of human hematopoietic stem cells in vitro and in vivo
Blood,
September 15, 2004;
104(6):
1662 - 1670.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Byrd, S. Becker, P. Maye, R. Narasimhaiah, B. St-Jacques, X. Zhang, J. McMahon, A. McMahon, and L. Grabel
Hedgehog is required for murine yolk sac angiogenesis
Development,
March 3, 2003;
129(2):
361 - 372.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Kuci, J. T. Wessels, H.-J. Buhring, K. Schilbach, M. Schumm, G. Seitz, J. Loffler, P. Bader, P. G. Schlegel, D. Niethammer, et al.
Identification of a novel class of human adherent CD34- stem cells that give rise to SCID-repopulating cells
Blood,
February 1, 2003;
101(3):
869 - 876.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Doyonnas, D. B. Kershaw, C. Duhme, H. Merkens, S. Chelliah, T. Graf, and K. M. McNagny
Anuria, Omphalocele, and Perinatal Lethality in Mice Lacking the Cd34-Related Protein Podocalyxin
J. Exp. Med.,
July 2, 2001;
194(1):
13 - 28.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. M. Felschow, M. L. McVeigh, G. T. Hoehn, C. I. Civin, and M. J. Fackler
The adapter protein CrkL associates with CD34
Blood,
June 15, 2001;
97(12):
3768 - 3775.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Prosper and C. M. Verfaillie
Regulation of hematopoiesis through adhesion receptors
J. Leukoc. Biol.,
March 1, 2001;
69(3):
307 - 316.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
S. Matsuoka, Y. Ebihara, M.-j. Xu, T. Ishii, D. Sugiyama, H. Yoshino, T. Ueda, A. Manabe, R. Tanaka, Y. Ikeda, et al.
CD34 expression on long-term repopulating hematopoietic stem cells changes during developmental stages
Blood,
January 15, 2001;
97(2):
419 - 425.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. D. Car and V. M. Eng
Special Considerations in the Evaluation of the Hematology and Hemostasis of Mutant Mice
Vet. Pathol.,
January 1, 2001;
38(1):
20 - 30.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. R. Beauchamp, L. Heslop, D. S.W. Yu, S. Tajbakhsh, R. G. Kelly, A. Wernig, M. E. Buckingham, T. A. Partridge, and P. S. Zammit
Expression of Cd34 and Myf5 Defines the Majority of Quiescent Adult Skeletal Muscle Satellite Cells
J. Cell Biol.,
December 11, 2000;
151(6):
1221 - 1234.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Doyonnas, J. Yi-Hsin Chan, L. H. Butler, I. Rappold, J. E. Lee-Prudhoe, A. C. W. Zannettino, P. J. Simmons, H.-J. Buhring, J.-P. Levesque, and S. M. Watt
CD164 Monoclonal Antibodies That Block Hemopoietic Progenitor Cell Adhesion and Proliferation Interact with the First Mucin Domain of the CD164 Receptor
J. Immunol.,
July 15, 2000;
165(2):
840 - 851.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. M. Watt, L. H. Butler, M. Tavian, H.-J. Buhring, I. Rappold, P. J. Simmons, A. C. W. Zannettino, D. Buck, A. Fuchs, R. Doyonnas, et al.
Functionally defined CD164 epitopes are expressed on CD34+ cells throughout ontogeny but display distinct distribution patterns in adult hematopoietic and nonhematopoietic tissues
Blood,
May 15, 2000;
95(10):
3113 - 3124.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. J. Drake and P. A. Fleming
Vasculogenesis in the day 6.5 to 9.5 mouse embryo
Blood,
March 1, 2000;
95(5):
1671 - 1679.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. A. Goodell
CD34+ or CD34-: Does it Really Matter?
Blood,
October 15, 1999;
94(8):
2545 - 2547.
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-i. Tada, M. Omine, T. Suda, and N. Yamaguchi
A Common Signaling Pathway Via Syk and Lyn Tyrosine Kinases Generated From Capping of the Sialomucins CD34 and CD43 in Immature Hematopoietic Cells
Blood,
June 1, 1999;
93(11):
3723 - 3735.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Korff and H. G. Augustin
Integration of Endothelial Cells in Multicellular Spheroids Prevents Apoptosis and Induces Differentiation
J. Cell Biol.,
November 30, 1998;
143(5):
1341 - 1352.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C.M. Verfaillie
Adhesion Receptors as Regulators of the Hematopoietic Process
Blood,
October 15, 1998;
92(8):
2609 - 2612.
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. M. Watt, H.-J. Buhring, I. Rappold, J. Y.-H. Chan, J. Lee-Prudhoe, T. Jones, A. C.W. Zannettino, P. J. Simmons, R. Doyonnas, D. Sheer, et al.
CD164, a Novel Sialomucin on CD34+ and Erythroid Subsets, Is Located on Human Chromosome 6q21
Blood,
August 1, 1998;
92(3):
849 - 866.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Gainsford, A. W. Roberts, S. Kimura, D. Metcalf, G. Dranoff, R. C. Mulligan, C. G. Begley, L. Robb, and W. S. Alexander
Cytokine Production and Function in c-mpl-Deficient Mice: No Physiologic Role for Interleukin-3 in Residual Megakaryocyte and Platelet Production
Blood,
April 15, 1998;
91(8):
2745 - 2752.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. C.-T. Hu and S. L. Chien
The Cytoplasmic Domain of Stem Cell Antigen CD34 Is Essential for Cytoadhesion Signaling But Not Sufficient for Proliferation Signaling
Blood,
February 15, 1998;
91(4):
1152 - 1162.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. M. McNagny, I. Pettersson, F. Rossi, I. Flamme, A. Shevchenko, M. Mann, and T. Graf
Thrombomucin, a Novel Cell Surface Protein that Defines Thrombocytes and Multipotent Hematopoietic Progenitors
J. Cell Biol.,
September 22, 1997;
138(6):
1395 - 1407.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. B. Wood, G. May, L. Healy, T. Enver, and G. M. Morriss-Kay
CD34 Expression Patterns During Early Mouse Development Are Related to Modes of Blood Vessel Formation and Reveal Additional Sites of Hematopoiesis
Blood,
September 15, 1997;
90(6):
2300 - 2311.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. L. Stanford, S. Haque, R. Alexander, X. Liu, A. M. Latour, H. R. Snodgrass, B. H. Koller, and P. M. Flood
Altered Proliferative Response by T Lymphocytes of Ly-6A (Sca-1) Null Mice
J. Exp. Med.,
August 29, 1997;
186(5):
705 - 717.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. G. Elefanty, L. Robb, R. Birner, and C. G. Begley
Hematopoietic-Specific Genes Are Not Induced During In Vitro Differentiation of scl-Null Embryonic Stem Cells
Blood,
August 15, 1997;
90(4):
1435 - 1447.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. J. Lawrence, C. D. Helgason, G. Sauvageau, S. Fong, D. J. Izon, R. K. Humphries, and C. Largman
Mice Bearing a Targeted Interruption of the Homeobox Gene HOXA9 Have Defects in Myeloid, Erythroid, and Lymphoid Hematopoiesis
Blood,
March 15, 1997;
89(6):
1922 - 1930.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
