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Suppression of apoptosis during cytokine deprivation of 32D cells is not
sufficient to induce complete granulocytic differentiation
JE Rodel and DC Link
Department of Medicine, Jewish Hospital, Washington University Medical
Center, St Louis, MO 63110, USA.
The role of cytokines in the control of hematopoietic cell differentiation
remains controversial. Two general models for the cytokine control of
hematopoietic differentiation have been proposed. In the stochastic model,
cytokines provide proliferative and survival signals to the differentiating
hematopoietic cell, but they do not provide specific lineage commitment
signals. In the instructive model, cytokines transmit specific signals to
multipotent hematopoietic cells, thereby directing lineage commitment. To
distinguish between these two models with respect to granulocyte
colony-stimulating factor (G-CSF) and granulocytic differentiation, we used
the 32Dcl3 cell line, which is capable of differentiating into granulocytes
in response to G-CSF, 32D cells transfected with either bcl-2 or bcl-XL
showed prolonged survival in medium containing no cytokine supplement.
Cells surviving in these cultures developed the segmented nuclei
characteristic of mature neutrophils. However, no induction of
myeloperoxidase activity or increase in cathepsin G transcripts were
detected. These data support a hybrid model for the role of G-CSF in
granulocytic differentiation; although some features of granulocytic
differentiation, namely nuclear segmentation, do not require G-CSF and
appear therefore to be preprogrammed in 32D cells, the complete maturation
of these cells to granulocytes appears to be dependent on G- CSF.
Volume 87,
Issue 3,
pp. 858-864,
02/01/1996
Copyright © 1996 by The American Society of Hematology
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