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Phenotypic, molecular, and functional characterization of human peripheral
blood CD34+/THY1+ cells
L Humeau, F Bardin, C Maroc, T Alario, R Galindo, P Mannoni and C Chabannon
Departement de Transfert de Gene et de Therapie Genique: Institut Paoli
Calmettes, Marseille, France.
A subset of mobilized CD34+ cells present in patient aphereses expresses
Thy1 (CDw90). This population contains most long-term culture initiating
cells, as assayed with a murine stromal cell line. It also contains a
significant proportion of colony-forming unit granulocyte macrophage, but
very few burst-forming unit erythroid. The limited differentiation towards
the erythroid lineage is further confirmed by the absence of GATA-1 mRNA in
the CD34+/Thy1+ subset, and by the low level of c-kit expression. The
CD34+/Thy1+ subset appears phenotypically and functionally heterogeneous, a
finding consistent with its high representation, compared to phenotypes
such as CD34+/CD38- . Therefore, while at least some of CD34+/Thy1+ cells
may be infectable by retroviral vectors, as shown by the presence of a
transcript for the receptor for murine amphotropic retroviruses, the use of
this selection strategy to specifically target human stem cells appears
questionable.
Volume 87,
Issue 3,
pp. 949-955,
02/01/1996
Copyright © 1996 by The American Society of Hematology
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