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Abundant expression of transcription factor GATA-2 in proliferating but not
in differentiated mast cells in tissues of mice: demonstration by in situ
hybridization
T Jippo, H Mizuno, Z Xu, S Nomura, M Yamamoto and Y Kitamura
Department of Pathology, Osaka University Medical School, Japan.
Although GATA-binding transcription factors (GATA-1 and GATA-2) are
strongly expressed in cultured mast cells (CMCs), their expression in mast
cells within tissues has not been reported. We examined the expression of
GATA-1 and GATA-2 in skin tissues of mice using Northern blot analysis and
in situ hybridization. mRNA for GATA-2 but not for GATA-1 was expressed in
skin mast cells of WB-+/+ embryos between days 15 and 17 postcoitum (pc).
The expression was downregulated on and after day 18 pc. Skin mast cells
did not express GATA-2 after birth either. When the number of skin mast
cells was compared with the number of GATA-2 mRNA-expressing cells, GATA-2
mRNA appeared to be expressed by mast cells only when the number was
increasing. When the mRNA expression of high-affinity IgE receptor
beta-subunit and mast cell carboxypeptidase A was used as differentiation
markers, the expression of these mRNAs continued even after the
downregulation of GATA-2 expression. To clarify the relationship of the
proliferation and GATA-2 expression, proliferating CMCs derived from
WBB6F1-+/+ mice were transplanted into the peritoneal cavity of mast
cell-deficient WBB6F1- W/Wv mice. The CMCs stopped both the proliferation
and GATA-2 expression after the transplantation, suggesting the association
of these two parameters in mast cells within tissues of mice.
Volume 87,
Issue 3,
pp. 993-998,
02/01/1996
Copyright © 1996 by The American Society of Hematology
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