TPA-induced arrest of erythroid differentiation is coupled with
downregulation of GATA-1 and upregulation of GATA-2 in an erythroid cell
line SAM-1
H Kamesaki, GY Michaud, SG Irving, N Suwabe, S Kamesaki, M Okuma and J Cossman
Laboratory of Experimental Radiology, Aichi Cancer Center Research
Institute, Nagoya, Japan.
GATA-1 protein is thought to be a positive regulator of erythroid
differentiation. However, ectopic expression of a conditional GATA-
2/estrogen receptor chimera was shown to inhibit erythroid differentiation
in a hormone-dependent manner, suggesting the negative regulation of
erythroid differentiation by GATA-2 protein. Accordingly, we reasoned that
the quantitative balance of GATA-1 and GATA-2 protein might affect
erythroid differentiation. In this report, we performed specific and
quantitative measurements of GATA-1 and GATA-2 protein in a new erythroid
cell line, SAM-1, after treatment with 12-O- tetradecanoylphorbol
13-acetate (TPA). On the basis of these measurements, we show that
TPA-induced arrest of erythroid differentiation is coupled with the
upregulation of GATA-2 protein, as well as the downregulation of GATA-1
protein. Our results suggest that it is the precise quantitative balance of
GATA-1 and GATA-2 protein that regulates erythroid differentiation.
Volume 87,
Issue 3,
pp. 999-1005,
02/01/1996
Copyright © 1996 by The American Society of Hematology
