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A monoclonal antibody recognizing CD43 (leukosialin) initiates apoptosis of
human hematopoietic progenitor cells but not stem cells
V Bazil, J Brandt, S Chen, M Roeding, K Luens, A Tsukamoto and R Hoffman
Research Department, SyStemix Inc, Palo Alto, CA 94304, USA.
CD43 (the major sialoglycoprotein of leukocytes) is an adhesion molecule
broadly expressed on hematopoietic cells. A monoclonal antibody recognizing
this molecule induces apoptosis of lineage marker- negative bone marrow
hematopoietic progenitor cells (HPCs) that express CD34 at a high density
(CD34hiLIN-). However, not all cells within this population undergo
apoptosis on stimulation via CD43. Dividing progenitor cells are most
highly affected, whereas more primitive quiescent cells survive anti-CD43
monoclonal antibody treatment. These surviving cells (1) are enriched for
cobblestone area-forming cells, (2) repopulate fragments for human fetal
bone implanted into C.B-17 scid/scid mice, (3) have a potential to
differentiate in vivo to myeloid and lymphoid cells, and (4) have a high
proliferative potential in long-term stromal cell-free liquid culture.
These data indicate that cells with hematopoietic stem cell characteristics
are relatively resistant to CD43-mediated apoptosis as compared with HPCs.
Thus, CD43 may be specifically involved in the regulation of HPC
proliferation.
Volume 87,
Issue 4,
pp. 1272-1281,
02/15/1996
Copyright © 1996 by The American Society of Hematology
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