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The eosinophil-specific cell surface antigen, EOS47, is a chicken homologue
of the oncofetal antigen melanotransferrin
KM McNagny, F Rossi, G Smith and T Graf
Differentiation Program, European Molecular Biology Laboratory, Heidelberg,
Germany.
The EOS47 antigen is a 100-kD cell surface glycoprotein selectively
expressed by avian retrovirus-transformed eosinophils and their precursors.
We have purified the EOS47 protein to homogeneity and used peptide sequence
information to clone EOS47-encoding cDNAs. The open reading frames from
these cDNAs predict a 738 amino acid protein with homology to human
melanotransferrin, a membrane-found, transferrin-like protein that is
expressed at high levels by a subset of melanomas, tumor cell lines, fetal
intestine, and liver, but not by most normal adult tissues. The predicted
protein sequence of EOS47 displays a 61% sequence identity with
melanotransferrin and conservation of all 28 cysteine residues, indicating
a similar tertiary structure. The finding that EOS47 lacks several of the
iron-coordinating amino acids present in all transferrins suggests that it
may be impaired in its ability to bind iron. In nonhematopoietic tissues,
EOS47 is expressed at high levels by epithelial brush borders of small
intestine and kidney and at lower levels by cells lining the sinusoids of
the liver. Within hematopoietic tissues, EOS47 is restricted to a
subpopulation of cells (1% to 5%) in bone marrow and early spleen and
fluorescence-activated cell sorting of EOS47+ cells leads to a dramatic (
> 30-fold) enrichment of peroxidase+ eosinophils. In contrast,
peripheral blood eosinophils are EOS47-, suggesting that the antigen is
expressed by newly formed eosinophils and that expression ceases shortly
before these cells emigrate from the bone marrow into the peripheral blood.
Our results show that melanotransferrin is a stage-specific marker of
eosinophils and should be useful for their isolation and further
characterization.
Volume 87,
Issue 4,
pp. 1343-1352,
02/15/1996
Copyright © 1996 by The American Society of Hematology
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